Current Position:Home>>Kits>>Metabolism Assays>> Glutathione Reductase (GR) Activity Assay Kit

Glutathione Reductase (GR) Activity Assay Kit

Cat.No.:E-BC-K099-S
Detection instrument: Spectrophotometer(340 nm)

Detection method: Colorimetric method

To Purchase E-BC-K099-S

Size:

100Assays
50Assays

Price: $200

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

With the coenzyme as hydrogen donor, GSSG can be reduced to GSH under the catalysis of GR. Then the GSH content increased and NADPH decreased. The decrease of NADPH absorbance can be measured at 340 nm. The activity of GR can be calculated by detecting the change of NADPH.

Performance characteristics

Synonyms	GR
Sample type	Serum,plasma,tissue,cells
Sensitivity	6.2 U/L
Detection range	6.2-320 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	90 min
Precision	Average inter-assay CV: 2.500%Average intra-assay CV: 2.100%
Other instruments required	Incubator, Vortex mixer, Micropipettor, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 1: 2-8℃, others: -20℃
Valid period	12 months

Images

The activity of GR was significantly lower (*P<0.05) in arsenic treated group and increased in arsenic + melatonin group (#P<0.05) comparing to control.

N Abdollahzade et al found that melatonin attenuated oxidative DNA damage in rats and attenuate chronic arsenic neurotoxicity. Glutathione reductase (GR) activity of rat brain tissue was determined using GR activity assay kit (E-BC-K099-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (6.2-320 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Mouse serum	1
Mouse plasma	1
Rat serum	1
Rat plasma	1
10% Mouse liver tissue homogenization	1-3
10% Epipremnum aureum tissue homogenization	1

Note: The diluent is double distilled water or MERGEFIELD 其他试剂 normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
In vitro and in vivo anticancer effects of syringic acid on colorectal cancer: Possible mechanistic view

DOI: 10.1016/j.cbi.2020.109337

PMID: 33548266
Sample: Cell sample
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
Prenatal exposure to environmental pro-oxidants induces mitochondria-mediated epigenetic changes: a cross-sectional pilot study

DOI: 10.1007/s11356-022-21059-3

PMID: 35633452
Sample: plasma
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

DOI: 10.1016/j.jep.2022.115306

PMID: 35443217
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

DOI: 10.1016/j.biopha.2020.109841

PMID: 31972360
Sample: Tissue homogenate
Environmental Research Communications (2022) IF: 3.237
Metal accumulation in muscle and oxidative stress response in the liver of juvenile Oreochromis niloticus from contaminated sediment under a simulation of increasing temperature

DOI: 10.1088/2515-7620/ac803d

Sample: liver
LIFE SCIENCES (2020) IF: 3.647
Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

DOI: 10.1016/j.lfs.2020.117584

PMID: 32220623
Sample: Tissue homogenate
LIFE SCIENCES (2020) IF: 3.647
Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

DOI: 10.1016/j.lfs.2020.118876

PMID: 33310035
Sample: cell
MOLECULAR BIOLOGY REPORTS (2021) IF: 2.316
Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease

DOI: 10.1007/s11033-021-06779-y

Sample: SH-SY5Y cell
Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

DOI: 10.1002/jez.2603

Sample: brain

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

p***tSubmitted [ Jan 13 2024 ]

Asked: Is this kit suitable for mouse sample, please let me know

adminSubmitted [ Jan 13 2024 ]

Answered: Our kit is non-species-specific and can detect mouse serum (plasma) samples.

O*************RSubmitted [ Jan 01 2024 ]

Asked: Is this kit suitable for plant sample, please let me know

adminSubmitted [ Jan 01 2024 ]

Answered: Our laboratory has verified that the green plant sample can be detected, and the measured value is 11.465U/gprot. There are differences between the samples. Theoretically, if the GR enzyme activity in the sample is higher than the sensitivity of the kit, it can be detected. It is recommended that you buy a small size kit to do a pre-test.

R**********nSubmitted [ Jul 13 2022 ]

Asked: Will the glutathione Reductase activity assay kit (E-BC-K099-S) work on an automated analyzer as an enzymatic reaction or other reaction type? Do you have a control for this test

adminSubmitted [ Jul 13 2022 ]

Answered: Sorry to inform you that our kits can not work on an automated analyzer and we don't have a control for this test.

... Show All Show Less

Cat. No	E-BC-K099-S
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha