Glutathione-S-Transferase (GST) Activity Assay Kit (E-BC-K278-S)
For research use only.
| Detection Principle | GST can catalyze the binding of reduced glutathione (GSH) to dinitrobenzene (CDNB) and the product have an absorption peak at 340 nm. The activity of GSH-ST can be calculated by measuring the increasing rate of absorbance at 340 nm. |
| Synonyms | GSH-ST |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (340 nm) |
| Research Area | Oxidative Stress , Liver And Renal Function |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1 U/L |
| Detection Range | 1-79 U/L |
| Precision | inter-assay CV: 4.3% | intra-assay CV: 1.9% |
| Sample Volume | 100 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum (plasma) | 1 |
| 10% Rat liver tissue homogenate | 150-200 |
| 10% Rat lung tissue homogenate | 8-12 |
| 10% Rat kidney tissue homogenate | 10-15 |
| 10% Plant tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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