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Glutathione-S-Transferase (GST) Activity Assay Kit

Cat.No.:E-BC-K278-S
Detection instrument: Spectrophotometer(340 nm)

Detection method: Colorimetric method

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Detection principle

GST can catalyze the binding of reduced glutathione (GSH) to dinitrobenzene (CDNB) and the product have an absorption peak at 340 nm. The activity of GSH-ST can be calculated by measuring the increasing rate of absorbance at 340 nm.

Performance characteristics

Synonyms	GSH-ST
Sample type	Serum,plasma,tissue,cells
Sensitivity	1 U/L
Detection range	1-79 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	50 min
Precision	Average inter-assay CV: 4.300%Average intra-assay CV: 1.900%
Other instruments required	Micropipettor, Incubator, Vortex mixer
Storage	2-8℃
Valid period	12 months

Images

GST activity was inhibited by adding in YPFS. (#P<0.05, ##P<0.01, *P<0.05, **P<0.01, ***P<0.001)

Y Du et al investigate the mechanism of Yu Ping Feng San in tracking the cisplatin-resistacne. Glutathione-S-Transferase (GST) avtivity in human cell was determined using GST activity assay kit (E-BC-K278-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and the results of the samples are linear within 5 min.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum (plasma)	1
10% Rat liver tissue homogenate	150-200
10% Rat lung tissue homogenate	8-12
10% Rat kidney tissue homogenate	10-15
10% Plant tissue homogenate	1

Note: The diluent is double distilled water or reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
In vitro and in vivo anticancer effects of syringic acid on colorectal cancer: Possible mechanistic view

DOI: 10.1016/j.cbi.2020.109337

PMID: 33548266
Sample: Cell sample
Frontiers in Pharmacology (2021) IF: 5.811
The Mechanisms of Yu Ping Feng San in Tracking the Cisplatin-Resistance by Regulating ATP-Binding Cassette Transporter and Glutathione S-Transferase in Lung Cancer Cells

DOI: 10.3389/fphar.2021.678126

PMID: 34135758
Sample: Cell culture supernatant
SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

DOI: 10.1016/j.sjbs.2022.103495

Sample: testicular tissue
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

DOI: 10.1016/j.jep.2022.115306

PMID: 35443217
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

DOI: 10.1016/j.biopha.2020.109841

PMID: 31972360
Sample: Tissue homogenate
Environmental Research Communications (2022) IF: 3.237
Metal accumulation in muscle and oxidative stress response in the liver of juvenile Oreochromis niloticus from contaminated sediment under a simulation of increasing temperature

DOI: 10.1088/2515-7620/ac803d

Sample: liver
LIFE SCIENCES (2020) IF: 3.647
Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

DOI: 10.1016/j.lfs.2020.117584

PMID: 32220623
Sample: Tissue homogenate
LIFE SCIENCES (2020) IF: 3.647
l-theanine alleviates liver and kidney dysfunction in septic rats induced by cecal ligation and puncture

DOI: 10.1016/j.lfs.2020.117502

PMID: 32142764
Sample: Tissue homogenate
LIFE SCIENCES (2020) IF: 3.647
Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

DOI: 10.1016/j.lfs.2020.118876

PMID: 33310035
Sample: tissue
Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

DOI: 10.1002/jez.2603

Sample: brain
BMC Veterinary Research (2022) IF: 2.741
Glutathione S-transferase (GST) and cortisol levels vs. microbiology of the digestive system of sheep during lambing

DOI: 10.1186/s12917-022-03201-y

PMID: 35303874
Sample: placenta
HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice:

DOI: 10.1177/09603271211045948

Sample: heart

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Reviews/Q&A

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Reviews
Q&A

N************RSubmitted [ Aug 05 2023 ]

Asked: Is this kit appropriate for studying with plants (lichens)?

adminSubmitted [ Aug 05 2023 ]

Answered: We'll reply to you by email. Pls pay attention to the mailbox

M**********dSubmitted [ Aug 26 2022 ]

Asked: What\'s the shelflife of this kit?

adminSubmitted [ Aug 26 2022 ]

Answered: 6 months.

D********dSubmitted [ Aug 04 2022 ]

Asked: E-BC-K278-S and E-BC-K800-M are both for GST determination, what\'s the difference?

adminSubmitted [ Aug 04 2022 ]

Answered: They both use DTNB as reagent but following different chromogenic systems, the detection wave length of E-BC-K278-S is 340 nm while it's 412 nm for E-BC-K800-M. Meanwhile, E-BC-K278-S uses spectrophotometer for measurement, and E-BC-K800-M uses microplate reader.

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