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Cat.No.:E-BC-K278-S
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Protocols for metabolism assays
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Synonyms | GSH-ST |
Sample type | Serum,plasma,tissue,cells |
Sensitivity | 1 U/L |
Detection range | 1-79 U/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 50 min |
Precision | Average inter-assay CV: 4.300%Average intra-assay CV: 1.900% |
Other instruments required | Micropipettor, Incubator, Vortex mixer |
Storage | 2-8℃ |
Valid period | 12 months |
GST activity was inhibited by adding in YPFS. (#P<0.05, ##P<0.01, *P<0.05, **P<0.01, ***P<0.001)
Y Du et al investigate the mechanism of Yu Ping Feng San in tracking the cisplatin-resistacne. Glutathione-S-Transferase (GST) avtivity in human cell was determined using GST activity assay kit (E-BC-K278-S).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and the results of the samples are linear within 5 min.
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum (plasma) |
1 |
10% Rat liver tissue homogenate |
150-200 |
10% Rat lung tissue homogenate |
8-12 |
10% Rat kidney tissue homogenate |
10-15 |
10% Plant tissue homogenate |
1 |
Note: The diluent is double distilled water or reagent 1.
PMID: 33548266 |
Sample: Cell sample |
PMID: 34135758 |
Sample: Cell culture supernatant |
Sample: testicular tissue |
PMID: 35443217 |
Sample: serum |
PMID: 31972360 |
Sample: Tissue homogenate |
Sample: liver |
PMID: 32220623 |
Sample: Tissue homogenate |
PMID: 32142764 |
Sample: Tissue homogenate |
PMID: 33310035 |
Sample: tissue |
DOI: 10.1002/jez.2603 |
|
Sample: brain |
PMID: 35303874 |
Sample: placenta |
Sample: heart |
N************RSubmitted [ Aug 05 2023 ]
Asked: Is this kit appropriate for studying with plants (lichens)?
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adminSubmitted [ Aug 05 2023 ]
Answered: We'll reply to you by email. Pls pay attention to the mailbox
M**********dSubmitted [ Aug 26 2022 ]
Asked: What\'s the shelflife of this kit?
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adminSubmitted [ Aug 26 2022 ]
Answered: 6 months.
D********dSubmitted [ Aug 04 2022 ]
Asked: E-BC-K278-S and E-BC-K800-M are both for GST determination, what\'s the difference?
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adminSubmitted [ Aug 04 2022 ]
Answered: They both use DTNB as reagent but following different chromogenic systems, the detection wave length of E-BC-K278-S is 340 nm while it's 412 nm for E-BC-K800-M. Meanwhile, E-BC-K278-S uses spectrophotometer for measurement, and E-BC-K800-M uses microplate reader.