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Glutathione-S-Transferase (GST) Activity Assay Kit

  • Cat.No.:E-BC-K278-S

  • Detection instrument: Spectrophotometer(340 nm)

To Purchase E-BC-K278-S

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Detection principle

GST can catalyze the binding of reduced glutathione (GSH) to dinitrobenzene (CDNB) and the product have an absorption peak at 340 nm. The activity of GSH-ST can be calculated by measuring the increasing rate of absorbance at 340 nm.

Performance characteristics

Synonyms GSH-ST
Sample type Serum,plasma,tissue,cells
Sensitivity 1 U/L
Detection range 1-79 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 50 min
Precision Average inter-assay CV: 4.3%Average intra-assay CV: 1.9%
Other instruments required Micropipettor, Incubator, Vortex mixer
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and the results of the samples are linear within 5 min.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum (plasma)

1

10% Rat liver tissue homogenate

150-200

10% Rat lung tissue homogenate

8-12

10% Rat kidney tissue homogenate

10-15

10% Plant tissue homogenate

1

Note: The diluent is double distilled water or reagent 1.

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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