Glutathione-S-Transferase (GST) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K278-S

      • 100Assays
      • 50Assays
      - +
      Price: $180
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(340 nm)

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      Detection principle

      GST can catalyze the binding of reduced glutathione (GSH) to dinitrobenzene (CDNB) and the product have an absorption peak at 340 nm. The activity of GSH-ST can be calculated by measuring the increasing rate of absorbance at 340 nm.

      Performance characteristics

      Synonyms GSH-ST
      Sample type Serum,plasma,tissue,cells
      Sensitivity 1 U/L
      Detection range 1-79 U/L
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 50 min
      Precision Average inter-assay CV: 4.3%Average intra-assay CV: 1.9%
      Other instruments required Micropipettor, Incubator, Vortex mixer
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and the results of the samples are linear within 5 min.

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum (plasma)


      10% Rat liver tissue homogenate


      10% Rat lung tissue homogenate


      10% Rat kidney tissue homogenate


      10% Plant tissue homogenate


      Note: The diluent is double distilled water or reagent 1.

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      MSDS for ELISA ISO 9001

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