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Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)

Cat.No.:E-BC-K073-S
Detection instrument: Spectrophotometer(620 nm)

Detection method: Colorimetric method

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Detection principle

Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.

Performance characteristics

Sample type	Animal liver,muscle
Sensitivity	1.80 mg/g liver tiss
Detection range	1.80-180 mg/g liver
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 7.300%Average intra-assay CV: 3.700%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), Concentrated sulfuric acid
Storage	2-8℃
Valid period	12 months

Images

The concentration of glycogen I liver and muscle were significantly changed in different group. (*P<0.05 vs. RC, #P<0.05 vs. SC)

YF Peng et al investigates the function of Lycium barbarum polysaccharide in regulating oxidative stress and energy metabolism in rat. Glycogen of rat liver and muscle was determined using glycogen colorimetric assay kit (E-BC-K073-S).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats

DOI: 10.3390/ijms231810920

PMID: 36142831
Sample: muscle,liver
ENDOCRINOLOGY (2023) IF: 5.051
Aspirin suppresses hepatic glucagon signaling through decreasing production of thromboxane A2

DOI: 10.1210/endocr/bqac217

Sample: Liver

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