Glycolate Oxidase Activity Assay Kit (E-BC-K692-S)
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For research use only.
| Detection Principle | Glycolate oxidase catalyzes sodium glycolate substrate to form glyoxylic acid, which reacts with phenylhydrazine hydrochloride to form phenylhydrazone glyoxalate. The substance has an absorption peak at 324 nm, and its OD value is proportional to the concentration of phenylhydrazone glyoxalate in a certain range, and the amount of phenylhydrazone generated reflects the activity of glycolate oxidase. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (324 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.3 U/mL |
| Detection Range | 0.3-350 U/mL |
| Precision | inter-assay CV: 8.2% | intra-assay CV: 1.1% |
| Sample Volume | 50 μL(tissue homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Oxalis corniculata tissue homogenate | 1 |
| 10% Ginkgo biloba tissue homogenate | 1 |
| 10% Cactus tissue homogenate | 1 |
| 10% Bamboo leaf tissue homogenate | 1 |
| 10% Osmanthus fragrans leaf tissue homogenate | 4-8 |
| 10% Camphor trees leaf tissue homogenate | 3-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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