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H2S Colorimetric Assay Kit

  • Cat.No.:E-BC-K355-M

  • Detection instrument: Microplate reader(660-670 nm,optimum wavelength: 为665 nm)

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Price: $220

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Detection principle

H2S can react with acetate solution to form ZnS which can be dissolved in alkaline solution. In the presence of Fe3+, methylene blue can be formed. Methylene blue has a maximum absorption peak at 665 nm. H2S content can be calculated indirectly by measuring the OD value at 665 nm.

Performance characteristics

Synonyms H2S
Sample type Serum,plasma,animal tissue
Sensitivity 6.73 μmol/L
Detection range 6.82-100 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 9.9%Average intra-assay CV: 3.1%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (6.82-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human plasma


Mouse plasma


10% Rat spleen tissue homogenate


10% Rat kidney tissue homogenate


10% Rat brain tissue homogenate


Note: The diluent is normal saline (0.9% NaCl).


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