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H2S Colorimetric Assay Kit

  • Cat.No.:E-BC-K355-M

  • Detection instrument: Microplate reader(660-670 nm,optimum wavelength: 为665 nm)

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  • 96T
  • 48T
Price: $220
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Detection principle

H2S can react with acetate solution to form ZnS which can be dissolved in alkaline solution. In the presence of Fe3+, methylene blue can be formed. Methylene blue has a maximum absorption peak at 665 nm. H2S content can be calculated indirectly by measuring the OD value at 665 nm.

Performance characteristics

Synonyms H2S
Sample type Serum,plasma,animal tissue
Sensitivity 6.73 μmol/L
Detection range 6.82-100 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 9.9%Average intra-assay CV: 3.1%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (6.82-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human plasma

1

Mouse plasma

1

10% Rat spleen tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat brain tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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