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H2S Colorimetric Assay Kit

Cat.No.:E-BC-K355-M
Detection instrument: Microplate reader(660-670 nm,optimum wavelength: 为665 nm)

Detection method: Colorimetric method

To Purchase E-BC-K355-M

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96T
48T

Price: $220

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Protocols for metabolism assays

FAQ for metabolism assays

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Detection principle

H2S can react with acetate solution to form ZnS which can be dissolved in alkaline solution. In the presence of Fe3+, methylene blue can be formed. Methylene blue has a maximum absorption peak at 665 nm. H2S content can be calculated indirectly by measuring the OD value at 665 nm.

Performance characteristics

Synonyms	H2S
Sample type	Serum,plasma,animal tissue
Sensitivity	2.75 μmol/L
Detection range	4.86-100 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	80 min
Precision	Average inter-assay CV: 9.900%Average intra-assay CV: 3.100%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Images

H2S level in cell is significantly increased by dosage.

Y Q Zhang et al developed a hydrogen sulphide-responsive and depleting nanoplatform for cancer photodynamic therapy. H2S level in cell was determined using H2S colorimetric assay kit (E-BC-K355-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (6.82-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
Mouse plasma	1
10% Rat spleen tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat brain tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Nature Communications (2022) IF: 14.919
A hydrogen sulphide-responsive and depleting nanoplatform for cancer photodynamic therapy

DOI: 10.1038/s41467-022-29284-7

PMID: 35354794
Sample: liver
TALANTA (2021) IF: 6.057
A dual lock-and-key two photon fluorescence probe in response to hydrogen peroxide and viscosity: Application in cellular imaging and inflammation therapy

DOI: 10.1016/j.talanta.2021.122719

PMID: 34517587
Sample: H9C2 cells
Cells (2022) IF: 6.6
Hyperglycemia and Loss of Redox Homeostasis in COVID-19 Patients

DOI: 10.3390/cells11060932

PMID: 35326383
Sample: plasma
NITRIC OXIDE-BIOLOGY AND CHEMISTRY (2021) IF: 4.427
The role of l-cysteine/Hydrogen sulfide pathway on β3-Adrenoceptor- induced relaxation in mouse gastric fundus

DOI: 10.1016/j.niox.2021.12.004

PMID: 34902580
Sample: gastric fundal tissue
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
Diabetic nephropathy associates with deregulation of enzymes involved in kidney sulphur metabolism

DOI: 10.1111/jcmm.15855

PMID: 32935914
Sample: Plasma,Tissue homogenate
International Braz J Urol (2022) IF: 3.05
Co-administration of sodium hydrosulfide and tadalafil modulates hypoxia and oxidative stress on bladder dysfunction in a rat model of bladder outlet obstruction.

DOI: 10.1590/s1677-5538.ibju.2022.0207

Sample: bladder tissue
NITRIC OXIDE-BIOLOGY AND CHEMISTRY (2019) IF: 3.371
Involvement of RhoA/Rho-kinase in l-cysteine/H2S pathway-induced inhibition of agonist-mediated corpus cavernosal smooth muscle contraction

DOI: 10.1016/j.niox.2019.02.001

PMID: 30768962
Sample: Tissue homogenate
NEUROUROLOGY AND URODYNAMICS (2020) IF: 2.037
Evaluation of combined therapeutic effects of hydrogen sulfide donor sodium hydrogen sulfide and phosphodiesterase type‐5 inhibitor tadalafil on erectile dysfunction in a partially bladder outlet obstructed rat model

DOI: 10.1002/nau.24333

Sample: Tissue homogenate
ANDROLOGIA (2019) IF: 1.84
Effects of hydrogen sulphide donor, sodium hydrosulphide treatment on the erectile dysfunction in L‐NAME‐induced hypertensive rats

DOI: 10.1111/and.13240

Sample: Tissue homogenate

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Reviews/Q&A

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Reviews
Q&A

E***********mSubmitted [ Jun 08 2023 ]

Asked: Is there a difference in the product size 48T and 96T in the quantity of the reagents? because i want to get the kit for a single experiment and won\'t be needing it anymore

adminSubmitted [ Jun 08 2023 ]

Answered: Hello Esraa, Thanks for your valuable question, for the product #E-BC-K355-M, if perform the experiment according to its product manual, the product size 48T allows to detect 31 samples, and the product size 96T allows to detect 79 samples. The product lead time is 1-2 business days. Please let us know if we could be of any further assistance :)

M*****iSubmitted [ Oct 13 2022 ]

Asked: Can the sample be detected if it exceeds the detection range written on the instruction manual?

adminSubmitted [ Oct 13 2022 ]

Answered: Yes, but please dilute the sample for detection.

M*****iSubmitted [ Aug 17 2022 ]

Asked: Is the sample of plant tissue available?

adminSubmitted [ Aug 17 2022 ]

Answered: Unfortunately, this kit is not suitable for plant tissues.

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