Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.

General Information

Product Form	Liquid
Concentration	1×
pH	7.2-7.4
D-Glucose	1802mg/L
HEPES	Negative
L-glutamine	1mM
NaHCO₃	1176mg/L
Phenol red	1.2mg/L
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	24 months

Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

Reviews/Q&A

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Reviews
Q&A

L*****aSubmitted [ Jan 28 2022 ]

Asked: Hello. We received Ham\'s F12 Medium in frozen form. Please, tell us, how bad is that? Can it be used for cell culture? Thanks.

adminSubmitted [ Jan 28 2022 ]

Answered: Hi Dear, There is no problem to keep the medium in frozen form. But it is important to make it melt throughly before experiment, as incomplete melt will cause the component in medium not to take effect during the experiment. Any other question, please feel free to contact us. Regards, Cathy

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