HMOX2 Polyclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human ;Mouse ;Rat
- Applications: WB
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:Human liver,HepG2,Jurkat,K562 |
Dilution |
WB 1:200-1:1000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant protein of human HMOX2 |
Abbre | HMOX2 |
Synonyms | Heme oxygenase (decycling) 2;Heme oxygenase (decyclizing) 2;Heme oxygenase 2;HMOX 2;Hmox2;HMOX2 protein;HMOX2;HO 2;HO-2;HO2;OTTHUMP00000159847 |
Swissprot | |
Calculated MW | 36kDa |
Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Microsome. Endoplasmic reticulum. |
Concentration | 0.6 mg/mL |
Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology; Metabolism |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus. |