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HSP27 Monoclonal Antibody

Cat:E-AB-22157
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:Hela
Verified Samples in IHC:Human breast carcinoma
Dilution

WB 1:1000-1:3000, IHC 1:50-1:200

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Recombinant Protein
Abbre HSP27
Synonyms Heat shock 27kDa protein;28 kDa heat shock protein;CMT2F;DKFZp586P1322;epididymis secretory protein Li 102;Estrogen regulated 24 kDa protein;Estrogen-regulated 24 kDa protein;Heat shock 25kDa protein 1;Heat shock 27 kDa protein;Heat shock 27kD protein 1;Heat shock 27kDa protein 1;Heat shock 28kDa protein 1;Heat Shock Protein 27;Heat shock protein beta 1;Heat shock protein beta-1;heat shock protein family B (small) member 1;HEL-S-102;HMN2B;HS.76067;Hsp 25;HSP 27;Hsp 28;Hsp B1;Hsp25;HSP27;Hsp28;HspB1;HSPB1;SRP27;Stress responsive protein 27;Stress-responsive protein 27
Swissprot
Observed MW 27kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Nucleus. Cytoplasm>cytoskeleton>spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Tissue Specificity Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method Protein A purification
Research Areas Cancer; Cardiovascular; Signal Transduction
Clone No. Clone:3E5
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Involved in stress resistance and actin organization.