HSP60 Polyclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC-p;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:LoVo,3T3,Rat muscle |
Dilution |
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Synthesized peptide derived from the C-terminal region of human HSP60 |
Abbre | HSP60 |
Synonyms | 60 kDa chaperonin;60 kDa heat shock protein;mitochondrial;CH60;Chaperonin 60;Chaperonin;60-KD;CPN60;fa04a05;GROEL;heat shock 60kDa protein 1 (chaperonin);Heat shock protein 1 (chaperonin);Heat shock protein 60;Heat shock protein 65;heat shock protein family D (Hsp60) member 1;HLD4;Hsp 60;HSP 65;HSP-60;HSP60;HSP65;HSPD1;HuCHA60;Mitochondrial matrix protein P1;P60 lymphocyte protein;short heat shock protein 60 Hsp60s1;SPG13 |
Swissprot | |
Calculated MW | 61kDa |
Observed MW |
68kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm |
Concentration | 1 mg/mL |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Signal Transduction; Tags and Cell Markers |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. |