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HSP60 Polyclonal Antibody

Cat:E-AB-31751
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p;IF
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Product Details
Verified Samples Verified Samples in WB:LoVo,3T3,Rat muscle
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from the C-terminal region of human HSP60
Abbre HSP60
Synonyms 60 kDa chaperonin;60 kDa heat shock protein;mitochondrial;CH60;Chaperonin 60;Chaperonin;60-KD;CPN60;fa04a05;GROEL;heat shock 60kDa protein 1 (chaperonin);Heat shock protein 1 (chaperonin);Heat shock protein 60;Heat shock protein 65;heat shock protein family D (Hsp60) member 1;HLD4;Hsp 60;HSP 65;HSP-60;HSP60;HSP65;HSPD1;HuCHA60;Mitochondrial matrix protein P1;P60 lymphocyte protein;short heat shock protein 60 Hsp60s1;SPG13
Swissprot
Calculated MW 61kDa
Observed MW 68kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Signal Transduction; Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.