Human IFN-β(Interferon Beta) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H0085

      Synonyms:IFNB1, IFB, IFF, IFNB

      • 96T
      • 24T
      - +
      Price: $495

      Reactivity: Human

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human IFN-β concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids


      This kit recognizes Human IFN-β in samples. No significant cross-reactivity or interference between Human IFN-β and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      O.D Average Corrected
      2000 2.51
      2.516 2.439
      1000 1.671
      1.698 1.621
      500 0.978
      0.963 0.886
      250 0.461
      0.467 0.39
      125 0.281
      0.272 0.195
      62.5 0.183
      0.178 0.101
      31.25 0.12
      0.129 0.052
      0 0.07
      0.077 --


      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IFN-β were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IFN-β were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 105.20 289.60 951.90 107.40 261.00 1001.00
      Standard deviation 5.90 14.20 40.00 5.40 11.70 54.10
      C V (%) 5.61 4.90 4.20 5.03 4.48 5.40


      The recovery of Human IFN-β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 88-102 93
      EDTA plasma (n=5) 92-106 98
      Cell culture media (n=5) 86-98 93


      Samples were spiked with high concentrations of Human IFN-β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 94-107 91-106 88-99
      Average (%) 101 97 94
      1:4 Range (%) 100-117 84-99 96-112
      Average (%) 107 90 103
      1:8 Range (%) 98-113 80-96 95-110
      Average (%) 104 87 102
      1:16 Range (%) 98-116 82-92 96-110
      Average (%) 106 87 103

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

      2.Remove the liquid.

      3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

      4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

      6.Add 50 μL Stop Solution.

      7.Read at 450 nm immediately. Calculation of results.


      1. Publication: Yang Q, Fu S, Wang J. Hepatitis C Virus Infection Decreases The Expression Of Toll-Like Receptors 3 And 7 Via Upregulation Of Mir-758[J]. Archives of Virology, 2014, 159(11): 2997-3003.
        Sample Type: Cell supernatant
      2. Publication: Peng D, Wang Z, Huang A, et al. A Novel Function Of F-Box Protein FBXO17 In Negative Regulation Of Type I IFN Signaling By Recruiting PP2A For IFN Regulatory Factor 3 Deactivation[J]. Journal of Immunology, 2016.
        Sample Type: culture supernatant
      3. Publication: Chen J, Xu W, Chen Y, et al. Matrix Metalloproteinase 9 Facilitates Hepatitis B Virus Replication Through Binding With IFNAR1 To Repress IFN/JAK/STAT Signaling[J]. Journal of Virology, 2017.
        Sample Type: culture supernatant
      4. Publication: Qian W, Wei X, Guo K, et al. The C-Terminal Effector Domain Of Non-Structural Protein 1 Of Influenza A Virus Blocks IFN-β Production By Targeting TNF Receptor-Associated Factor 3[J]. Frontiers in Immunology, 2017.
        Sample Type: culture supernatant
      5. Publication: Yi C Y, Zhao Z Z, Wang S Y, et al. Influenza A Virus Pa Antagonizes Interferon-β By Interacting With Interferon Regulatory Factor 3[J]. Frontiers in Immunology, 2017.
        Sample Type: culture supernatant
      6. Publication: Dai J P, Gu L M, Su Y, et al. Inhibition Of Curcumin On Influenza A Virus Infection And Influenzal Pneumonia Via Oxidative Stress, TLR2/4, p38/JNK MAPK And NF-κB Pathways[J]. International Immunopharmacology, 2018, 54: 177-187.
        Sample Type: culture supernatant
      7. Publication: Wang K Y, Lai C C, Gu H J, et al. miR-194 Inhibits Innate Antiviral Immunity By Targeting FGF2 In Influenza H1N1 Virus Infection[J]. Frontiers in Microbiology, 2017.
        Sample Type: Culture supernatant and bronchoalveolar lavage flu
      8. Publication: Chen J B, Xu W, Chen Y N, et al. MMP-9 Facilitates Hepatitis B Virus Replication Through Binding With IFNAR1 To Repress IFN/JAK/STAT Signaling[J]. Journal of Virology, 2017.
        Sample Type: culture supernatant
      9. Publication: Wang Z Y , Sheng C J , Yao C , et al. The EF-Hand Protein CALML6 Suppresses Antiviral Innate Immunity by Impairing IRF3 Dimerization[J]. Cell Reports , 2019, 26: 1273–1285.
        Sample Type: Cell supernatants
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