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Human IL-4(Interleukin 4) ELISA Kit

Citations(26)Uniprot : P05112

  • Cat.No.:E-EL-H0101

  • Reactivity: Human
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-4 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-4. You can calculate the concentration of Human IL-4 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-4 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 102.80 203.60 960.70 107.70 191.50 872.50
Standard deviation 6.20 8.60 33.60 6.60 9.60 26.20
CV (%) 6.03 4.22 3.50 6.13 5.01 3.00

Recovery

The recovery of Human IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma (n=8) 93-108 98
Cell culture media (n=8) 90-104 96

Linearity

Samples were spiked with high concentrations of Human IL-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P05112
SynonymsIL4, BCGF-1, BCGF1, BSF-1, BSF1
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    		 Sample: Tissue homogenate
  2. CANCER LETTERS (2022) IF: 8.679
    Decreased CXCR2 expression on circulating monocytes of colorectal cancer impairs recruitment and induces Re-education of tumor-associated macrophages

    DOI: 10.1016/j.canlet.2022.01.004

    PMID: 34999169

    		 Sample: serum,THP-1 cell,human circulating CD14+ monocytes
  3. Antioxidants (2022) IF: 7.675
    Genipin Attenuates Diabetic Cognitive Impairment by Reducing Lipid Accumulation and Promoting Mitochondrial Fusion via FABP4/Mfn1 Signaling in Microglia

    DOI: 10.3390/antiox12010074
    Sample: HMC3 cells
  4. Frontiers in Nutrition (2022) IF: 6.59
    Different oral and gut microbial profiles in those with Alzheimer's disease consuming anti-inflammatory diets.

    DOI: 10.3389/fnut.2022.974694
    Sample: serum
  5. JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    		 Sample: Cell culture supernatant
  6. WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742
    Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

    DOI: 10.3748/wjg.v27.i38.6489

    PMID: 34720537

    		 Sample: serum
  7. Frontiers in Pharmacology (2022) IF: 5.811
    Berberine Inhibits the Adhesion of Candida albicans to Vaginal Epithelial Cells

    DOI: 10.3389/fphar.2022.814883

    PMID: 35295335

    		 Sample: vaginal epithelial cell
  8. Clinical and Translational Allergy (2022) IF: 5.871
    MiR-29b regulates Th2 cell differentiation in asthma by targeting inducible B7-H3 and STAT3

    DOI: 10.1002/clt2.12114

    PMID: 35079347

    		 Sample: THP‐1 cell
  9. Frontiers in Pharmacology (2022) IF: 5.988
    Metabolomics Profiles Associated with the Treatment of Zuojin Pill on Patients with Chronic Nonatrophic Gastritis

    DOI: 10.3389/fphar.2022.898680

    PMID: 35899115

    		 Sample: serum
  10. CLINICAL AND EXPERIMENTAL IMMUNOLOGY (2022) IF: 4.33
    Clinical features and nasal inflammation in asthma and allergic rhinitis

    DOI: 10.1093/cei/uxac019
    Sample: blood
  11. INFLAMMATION RESEARCH (2021) IF: 4.575
    Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis

    DOI: 10.1007/s00011-021-01459-w

    PMID: 33852061

    		 Sample: Serum,Bronchoalveolar Lavage Fluid
  12. INTERNATIONAL JOURNAL OF CARDIOLOGY (2016) IF: 4.638
    Decreased expression of programmed death 1 on peripheral blood lymphocytes disrupts immune homeostasis in peripartum cardiomyopathy

    DOI: 10.1016/j.ijcard.2016.08.232
    Sample: Serum
  13. Frontiers in Immunology (2019) IF: 4.716
    Severe Fever With Thrombocytopenia Syndrome Virus-Induced Macrophage Differentiation Is Regulated by miR-146

    DOI: 10.3389/fimmu.2019.01095
    Sample: serum
  14. INTERNATIONAL IMMUNOPHARMACOLOGY (2018) IF: 3.118
    Novel anti-inflammatory target of geniposide: Inhibiting Itgβ1/Ras-Erk1/2 signal pathway via the miRNA-124a in rheumatoid arthritis synovial fibroblasts

    DOI: 10.1016/j.intimp.2018.09.049
    Sample: MH7A cells
  15. Biomed Research International (2022) IF: 3.246
    Regulatory Effects of Fyn on Trophoblast Cell Behaviors and Function

    DOI: 10.1155/2022/6006981

    PMID: 36330453

    		 Sample: HTR-8/SVneo cells,JEG-3 cells
  16. Bioengineered (2021) IF: 3.269
    Coordination of PRKCA/PRKCA-AS1 interplay facilitates DNA methyltransferase 1 recruitment on DNA methylation to affect protein kinase C alpha transcription in mitral valve of rheumatic heart disease

    DOI: 10.1080/21655979.2021.1971482

    PMID: 34482802

    		 Sample: serum
  17. TUMOR BIOLOGY (2017) IF: 3.65
    PKCζ in prostate cancer cells represses the recruitment and M2 polarization of macrophages in the prostate cancer microenvironment:

    DOI: 10.1177/1010428317701442
    Sample: Cell culture supernatant,Cell lysate
  18. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 3.657
    Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?

    DOI: 10.1089/jir.2022.0098

    PMID: 36179036

    		 Sample: fecal
  19. Infectious Agents and Cancer (2023) IF: 3.698
    Inhibitory effect and mechanism of Lactobacillus crispatus on cervical precancerous cells Ect1/E6E7 and screening of early warning factors

    DOI: 10.1186/s13027-023-00483-1
    Sample: Ect1/E6E7 cell
  20. Annals of Translational Medicine (2022) IF: 3.932
    Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF

    DOI: 10.21037/atm-21-6812
    Sample: adipose-derived stem cell (ASCs)
  21. PLoS Neglected Tropical Diseases (2016) IF: 3.948
    Clonorchis sinensis Co-infection Could Affect the Disease State and Treatment Response of HBV Patients

    DOI: 10.1371/journal.pntd.0004806
    Sample: Serum,Cell culture supernatant
  22. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2019) IF: 2.086
    In vitro impact of bisphenol A on maturation and function of monocyte-derived dendritic cells in patients with primary Sjögren’s syndrome

    DOI: 10.1080/08923973.2019.1706554
    Sample: PBMCs
  23. CLINICAL RHEUMATOLOGY (2020) IF: 2.394
    The clinical value of interleukins-8, -10, and -17 in idiopathic granulomatous mastitis

    DOI: 10.1007/s10067-020-04925-8
    Sample: blood
  24. CLINICAL CARDIOLOGY (2016) IF: 2.431
    Expression of programmed cell death‐1 and its ligand B7 homolog 1 in peripheral blood lymphocytes from patients with peripartum cardiomyopathy

    DOI: 10.1002/clc.22661
    Sample: Serum
  25. American Journal of Rhinology & Allergy (2021) IF: 2.467
    Immunosuppressive Regulation of Dendritic Cells and T Cells in Allergic Rhinitis by Semaphorin 3A:

    DOI: 10.1177/19458924211005592
    Sample: Cell
  26. ORL-Journal for Oto-Rhino-Laryngology Head and Neck Surgery (2021) IF: 1.538
    Clinical Efficacy and Possible Mechanism of Endoscopic Vidian Neurectomy for House Dust Mite-Sensitive Allergic Rhinitis

    DOI: 10.1159/000511711
    Sample: serum
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