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Human IL-4(Interleukin 4) ELISA Kit

Citations(13)Uniprot : P05112

Cat.No.:E-EL-H0101
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human IL-4 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-4. You can calculate the concentration of Human IL-4 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-4 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	102.80	203.60	960.70	107.70	191.50	872.50
Standard deviation	6.20	8.60	33.60	6.60	9.60	26.20
CV (%)	6.03	4.22	3.50	6.13	5.01	3.00

Recovery

The recovery of Human IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-97	92
EDTA plasma (n=8)	93-108	98
Cell culture media (n=8)	90-104	96

Linearity

Samples were spiked with high concentrations of Human IL-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05112
Synonyms	IL4, BCGF-1, BCGF1, BSF-1, BSF1
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Theranostics (2021) IF: 11.556

A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

DOI: 10.7150/thno.58321
PMID: 33995679	Sample: Tissue homogenate

International Journal of Cardiology (2016) IF: 6.189

Decreased Expression Of Programmed Death 1 On Peripheral Blood Lymphocytes Disrupts Immune Homeostasis In Peripartum Cardiomyopathy.

DOI: 10.1016/j.ijcard.2016.08.232
PMID: 27580218	Sample: Serum

Journal of Cellular Physiology (2020) IF: 5.546

Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

DOI: 10.1002/jcp.29713
PMID: 32385929	Sample: Cell culture supernatant

ASSESSMENT (2018) IF: 3.197

Assessment of vitamin d therapy effect on inflammatory markers in pediatric patients with type i diabetic

DOI: 10.22159/ajpcr.2018.v11i10.28936
	Sample: Serum

Tumor Biology (2017) IF: 3.65

Pkcζ In Prostate Cancer Cells Represses The Recruitment And M2 Polarization Of Macrophages In The Prostate Cancer Microenvironment.

DOI: 10.1177/1010428317701442
PMID: 28631559	Sample: Cell culture supernatant,Cell lysate

Plos Neglected Tropical Diseases (2016) IF: 3.834

Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients.

DOI: 10.1371/journal.pntd.0004806
PMID: 27348302	Sample: Serum,Cell culture supernatant

Journal of Zhejiang University. Science. B (2020) IF: 2.082

Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China

DOI: 10.1631/jzus.B2000174
PMID: 32425003	Sample: Serum,Plasma

Clinical Cardiology (2016) IF: 2.757

Expression Of Programmed Cell Death-1 And Its Ligand B7 Homolog 1 In Peripheral Blood Lymphocytes From Patients With Peripartum Cardiomyopathy.

DOI: 10.1002/clc.22661
PMID: 28026044	Sample: Serum

International Immunopharmacology (2015) IF: 2.956

Intranasal Administration Of CpG Oligodeoxynucleotides Reduces Lower Airway Inflammation In A Murine Model Of Combined Allergic Rhinitis And Asthma Syndrome.

DOI: 10.1016/j.intimp.2015.06.028
PMID: 26163938	Sample: Bronchoalveolar Lavage Fluid

International Journal of Clinical and Experimental Pathology (2017) IF: 1.706

Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis.


	Sample: Cell culture supernatant

Biomedical and Pharmacology Journal (2016)

Microbial Index and TNF-α, IL-4, CCL17 Level Among Burn Wound Infections in Hilla City, Iraq.

DOI: 10.13005/bpj/1054
	Sample: Serum

International Electronic Journal of Medicine (2020)

Hematological Changes, Serum Interferon Gamma and Interleukin-4 Alterations in Normal Pregnancy and Preeclampsia

DOI: 10.34172/iejm.2020.08
	Sample: Serum

JOURNAL OF THI-QAR SCIENCE (2018)

The some cytokines Levels (TGFβ1, IL-4, IL-6, and IL-17) in sera Patients with Diabetes Mellitus Type1, Type 2 in Nassiriya city


	Sample: Serum

Kerbala Jorunal of Medicine (2017) IF: /

Role of IL-4 and Glutathione Peroxidase in Patients with Obstructive Lung Diseases.


	Sample: Serum

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