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Human IL-4(Interleukin 4) ELISA Kit

  • Cat.No.:E-EL-H0101

  • Reactivity: Human

To Purchase E-EL-H0101

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-4 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-4. You can calculate the concentration of Human IL-4 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-4 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 102.80 203.60 960.70 107.70 191.50 872.50
Standard deviation 6.20 8.60 33.60 6.60 9.60 26.20
CV (%) 6.03 4.22 3.50 6.13 5.01 3.00


The recovery of Human IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma (n=8) 93-108 98
Cell culture media (n=8) 90-104 96


Samples were spiked with high concentrations of Human IL-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P05112
SynonymsIL4, BCGF-1, BCGF1, BSF-1, BSF1
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    Sample: Tissue homogenate
  2. International Journal of Cardiology (2016) IF: 6.189
    Decreased Expression Of Programmed Death 1 On Peripheral Blood Lymphocytes Disrupts Immune Homeostasis In Peripartum Cardiomyopathy.

    DOI: 10.1016/j.ijcard.2016.08.232

    PMID: 27580218

    Sample: Serum
  3. Journal of Cellular Physiology (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  4. ASSESSMENT (2018) IF: 3.197
    Assessment of vitamin d therapy effect on inflammatory markers in pediatric patients with type i diabetic

    DOI: 10.22159/ajpcr.2018.v11i10.28936

    Sample: Serum
  5. Tumor Biology (2017) IF: 3.65
    Pkcζ In Prostate Cancer Cells Represses The Recruitment And M2 Polarization Of Macrophages In The Prostate Cancer Microenvironment.

    DOI: 10.1177/1010428317701442

    PMID: 28631559

    Sample: Cell culture supernatant,Cell lysate
  6. Plos Neglected Tropical Diseases (2016) IF: 3.834
    Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients.

    DOI: 10.1371/journal.pntd.0004806

    PMID: 27348302

    Sample: Serum,Cell culture supernatant
  7. Journal of Zhejiang University. Science. B (2020) IF: 2.082
    Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China

    DOI: 10.1631/jzus.B2000174

    PMID: 32425003

    Sample: Serum,Plasma
  8. Clinical Cardiology (2016) IF: 2.757
    Expression Of Programmed Cell Death-1 And Its Ligand B7 Homolog 1 In Peripheral Blood Lymphocytes From Patients With Peripartum Cardiomyopathy.

    DOI: 10.1002/clc.22661

    PMID: 28026044

    Sample: Serum
  9. International Immunopharmacology (2015) IF: 2.956
    Intranasal Administration Of CpG Oligodeoxynucleotides Reduces Lower Airway Inflammation In A Murine Model Of Combined Allergic Rhinitis And Asthma Syndrome.

    DOI: 10.1016/j.intimp.2015.06.028

    PMID: 26163938

    Sample: Bronchoalveolar Lavage Fluid
  10. International Journal of Clinical and Experimental Pathology (2017) IF: 1.706
    Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis.
    Sample: Cell culture supernatant
  11. Biomedical and Pharmacology Journal (2016)
    Microbial Index and TNF-α, IL-4, CCL17 Level Among Burn Wound Infections in Hilla City, Iraq.

    DOI: 10.13005/bpj/1054

    Sample: Serum
  12. International Electronic Journal of Medicine (2020)
    Hematological Changes, Serum Interferon Gamma and Interleukin-4 Alterations in Normal Pregnancy and Preeclampsia

    DOI: 10.34172/iejm.2020.08

    Sample: Serum
    The some cytokines Levels (TGFβ1, IL-4, IL-6, and IL-17) in sera Patients with Diabetes Mellitus Type1, Type 2 in Nassiriya city
    Sample: Serum
  14. Kerbala Jorunal of Medicine (2017) IF: /
    Role of IL-4 and Glutathione Peroxidase in Patients with Obstructive Lung Diseases.
    Sample: Serum
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