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Human ITLN1(Intelectin 1/Omentin) ELISA Kit

Citations(9)Uniprot : Q8WWA0

Cat.No.:E-EL-H2028
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.63-40 ng/mL
Sensitivity	0.38 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human ITLN1 in samples. No significant cross-reactivity or interference between Human ITLN1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human ITLN1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ITLN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ITLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ITLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ITLN1. You can calculate the concentration of Human ITLN1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ITLN1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ITLN1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.98	6.33	16.56	1.83	6.51	17.00
Standard deviation	0.12	0.27	0.67	0.12	0.30	0.53
CV (%)	6.06	4.27	4.05	6.56	4.61	3.12

Recovery

The recovery of Human ITLN1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-110	101
EDTA plasma (n=8)	89-105	96
Cell culture media (n=8)	84-98	91

Linearity

Samples were spiked with high concentrations of Human ITLN1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q8WWA0
Synonyms	HL-1, HL1, INTL, ITLN, LFR, hIntL, Intelectin 1,Galactofuranose Binding, Intestinal Lactoferrin Receptor
Research Area	Cancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cardiovascular Diabetology (2020) IF: 7.332
Association between omentin-1 and major cardiovascular events after lower extremity endovascular revascularization in diabetic patients: a prospective cohort study

DOI: 10.1186/s12933-020-01151-z

Sample: Serum
MECHANISMS OF AGEING AND DEVELOPMENT (2022) IF: 5.432
Sortilin/Omentin-1 ratio in peripheral artery disease: A cross-sectional study on 295 unselected elderly patients

DOI: 10.1016/j.mad.2022.111677

PMID: 35500800
Sample: serum
Frontiers in Pharmacology (2022) IF: 5.811
Effect of Ibandronate Therapy on Serum Chemerin, Vaspin, Omentin-1 and Osteoprotegerin (OPG) in Postmenopausal Osteoporotic Females.

DOI: 10.3389/fphar.2022.822671

Sample: serum
Cardiovascular Diabetology (2019) IF: 5.948
Association between plasma omentin-1 levels in type 2 diabetic patients and peripheral artery disease

DOI: 10.1186/s12933-019-0880-7

Sample: serum
JOURNAL OF DERMATOLOGICAL TREATMENT (2021) IF: 3.359
Effects of TNF inhibitors and an IL12/23 inhibitor on changes in body weight and adipokine levels in psoriasis patients: A 48-week comparative study

DOI: 10.1080/09546634.2021.1901845

PMID: 33704001
Sample: Serum
Dermatologic Therapy (2020) IF: 2.327
Correlation of metabolic syndrome with serum omentin‐1 and visfatin levels and disease severity in psoriasis and psoriatic arthritis

DOI: 10.1111/dth.14378

Sample: Serum
ACTA DIABETOLOGICA (2019) IF: 2.996
Placental expressions and serum levels of adiponectin, visfatin, and omentin in GDM

DOI: 10.1007/s00592-019-01355-0

Sample: serum
JOURNAL OF ASTHMA (2017) IF: 1.746
Relationship of circulating chemerin and omentin levels with Th17 and Th9 cell immune responses in patients with asthma

DOI: 10.1080/02770903.2017.1355378

Sample: Plasma
Turkish Journal of Pharmaceutical Sciences (2022)
Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis

DOI: 10.4274/tjps.galenos.2021.52284

Sample: serum

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