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Current Position:Home>>Kits>>ELISA Kits>> Human PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit

Human PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit

Citations(12)

  • Cat.No.:E-EL-H0185

  • Reactivity: Human
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human PⅠNP in samples. No significant cross-reactivity or interference between Human PⅠNP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human PⅠNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅠNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅠNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅠNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅠNP. You can calculate the concentration of Human PⅠNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 12 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 12 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PⅠNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PⅠNP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.12 128.38 437.51 54.81 139.67 400.58
Standard deviation 3.25 6.02 18.51 3.47 6.13 17.95
CV (%) 6.24 4.69 4.23 6.33 4.39 4.48

Recovery

The recovery of Human PⅠNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 90
EDTA plasma (n=8) 88-101 94
Cell culture media (n=8) 89-103 95

Linearity

Samples were spiked with high concentrations of Human PⅠNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsP1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide
Research AreaSignal Transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
    Biocompatible Gas Plasma Treatment Affects Secretion Profiles but Not Osteogenic Differentiation in Patient-Derived Mesenchymal Stromal Cells

    DOI: 10.3390/ijms23042038

    PMID: 35216160

    		 Sample: Bone Marrow Mesenchymal Stromal Cell
  2. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
    Maternal omega-3 LC-PUFA supplementation programs an improved bone mass in the offspring with a more pronounced effect in females than males at adulthood

    DOI: 10.1016/j.jnutbio.2022.109245
    Sample: Plasma
  3. International Journal of Endocrinology and Metabolism (2020)
    Effect of Vitamin D Levels on Bone Remodeling in Healthy Women

    DOI: 10.5812/ijem.100656

    PMID: 32636886

    		 Sample: Serum
  4. Bosnian Journal of Basic Medical Sciences (2018) IF: 1.432
    Evaluation of bone mineral density (BMD) and indicators of bone turnover in patients with hemophilia

    DOI: 10.17305/bjbms.2018.2335
    Sample: Serum
  5. Experimental and Therapeutic Medicine (2019) IF: 1.448
    Efficacy of vitamin D plus calcium with/without alendronate on bone metabolism in immunologic thrombocytopenic purpura patients with steroid treatment: Nine‑month results of a randomized, double‑blinded, controlled trial

    DOI: 10.3892/etm.2019.7694
    Sample: Serum,Urine
  6. Biomed Research International (2020) IF: 2.276
    Osteokines and Bone Markers at Rest and following Plyometric Exercise in Pre- and Postmenopausal Women

    DOI: 10.1155/2020/7917309

    PMID: 33145358

    		 Sample: Serum
  7. Biomed Research International (2018) IF: 2.583
    Response of Sclerostin and Bone Turnover Markers to High Intensity Interval Exercise in Young Women: Does Impact Matter?

    DOI: 10.1155/2018/4864952
    Sample: Serum
  8. Archives of Osteoporosis (2021) IF: 2.617
    Age-related trends and reference intervals of cross-linked C-telopeptide of type I collagen and procollagen type I N-propeptide from a reference population of Sri Lankan adult women

    DOI: 10.1007/s11657-021-01022-4

    PMID: 34727246

    		 Sample: Serum
  9. Archives of Osteoporosis (2021) IF: 2.617
    Age-related changes and reference intervals of RANKL, OPG, and bone turnover markers in Indian women

    DOI: 10.1007/s11657-021-01014-4

    PMID: 34606009

    		 Sample: Serum
  10. Pain Physician (2020) IF: 3.251
    Observational Study to Evaluate the Effect of Epidural Steroid Injection on Bone Mineral Density and Bone Turnover Markers.

    PMID: 32967402

    		 Sample: Serum
  11. CALCIFIED TISSUE INTERNATIONAL (2019) IF: 3.265
    Effects of High-Intensity Interval Running Versus Cycling on Sclerostin, and Markers of Bone Turnover and Oxidative Stress in Young Men

    DOI: 10.1007/s00223-019-00524-1

    PMID: 30671591

    		 Sample: Serum
  12. BONE (2018) IF: 4.455
    Bone and plasma citrate is reduced in osteoporosis

    DOI: 10.1016/j.bone.2018.06.014
    Sample: Serum
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

D************ Submitted [ May 15 2023 ]

Asked: I have a similar doubt as above.I analysed P1NP values in 90 patients using your kits. My doubt is previous studies have given normal values of P1NP around 25-80ng/ml. Where as your respective kits is standardised in range of 15- 1000pg/ml. Please explain what is the reason for this. Should we multiply our final result by 500, as dilution factor??please elaborate.

Reply

A

adminSubmitted [ May 15 2023 ]

Answered: Hello Dr Gopinath N,thank you for your feedback.We will reply you via E-mail.Please check your mailbox.

Q

G********* Submitted [ May 13 2023 ]

Asked: My question is similar to above one of P1NP kits. After running my samples all of my results were in 100-700pg/ml range , which is very low compared to existing normative data. As explained by your side earlier that normal samples were diluted by 500 times, should we multiply our end results with the same dilution factor, to get our results appropriately.

Reply

A

adminSubmitted [ May 13 2023 ]

Answered: Hello Gopinath N,thank you for your support. If your sample was diluted before testing, multiply it by the appropriate dilution in the final calculation. In addition, if you still have doubts about the test result, you can provide the standard curve tested, the original data of the sample and the lot number information of the kit, which will be used for further analysis by us. Please feel free to email to customer03@elabscience.com.

Q

P***lSubmitted [ Aug 28 2018 ]

Asked: I did notice that the assay kit for P1NP (Procollagen type 1 N-terminal propeptide) reports a very low detection range: it only detects values between 15.63 - 1000 pg/mL, or otherwise could be stated as 0.015 - 1 ng/mL. However, Population normal ranges for serum P1NP are: 15 - 80 ng/mL (I\'ve attached a poster reporting those population norms; the associated reference is Jenkins et al. 2013, Bone 55:271-276). Has the company made a typing error on the assay kit insert and used incorrect units or is that the true very low limits of detection for that kit?

Reply

A

adminSubmitted [ Aug 28 2018 ]

Answered: Thanks for your question. Referred from our in-house data, the normal serum samples were diluted in 500 folds, and the serum PINP levels were 40-110ng/mL, which consistents with the reference values in publications. If the concentration of the target protein in the sample is out of the highest detection limit, it just needs to be diluted to fall in the detection range. We usually try highest sensitivity to make the kits more flexible, so the kit could meet different experiments requirements. Further inquiry, please tell us.

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