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Human PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit

  • Cat.No.:E-EL-H0185

  • Reactivity: Human

To Purchase E-EL-H0185

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human PⅠNP in samples. No significant cross-reactivity or interference between Human PⅠNP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human PⅠNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅠNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅠNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅠNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅠNP. You can calculate the concentration of Human PⅠNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PⅠNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PⅠNP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.12 128.38 437.51 54.81 139.67 400.58
Standard deviation 3.25 6.02 18.51 3.47 6.13 17.95
CV (%) 6.24 4.69 4.23 6.33 4.39 4.48

Recovery

The recovery of Human PⅠNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 90
EDTA plasma (n=8) 88-101 94
Cell culture media (n=8) 89-103 95

Linearity

Samples were spiked with high concentrations of Human PⅠNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsP1NP, PINP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Bone (2018) IF: 4.455
    Bone and plasma citrate is reduced in osteoporosis.

    DOI: 10.1016/j.bone.2018.06.014

    PMID: 29929041

    Sample: serum
  2. Pain physician (2020) IF: 3.251
    Observational Study to Evaluate the Effect of Epidural Steroid Injection on Bone Mineral Density and Bone Turnover Markers

    PMID: 32967402

    Sample: Serum
  3. Calcified tissue international (2019) IF: 3.293
    Effects of High-Intensity Interval Running Versus Cycling on Sclerostin, and Markers of Bone Turnover and Oxidative Stress in Young Men

    DOI: 10.1007/s00223-019-00524-1

    PMID: 30671591

    Sample: Serum
  4. International Journal of Endocrinology and Metabolism (2020) IF: 1.962
    Effect of Vitamin D Levels on Bone Remodeling in Healthy Women

    DOI: 10.5812/ijem.100656

    PMID: 32636886

    Sample: Serum
  5. Experimental and therapeutic medicine (2019) IF: 1.785
    Efficacy of vitamin D plus calcium with/without alendronate on bone metabolism in immunologic thrombocytopenic purpura patients with steroid treatment: Nine-month results of a randomized, double-blinded, controlled trial

    DOI: 10.3892/etm.2019.7694

    PMID: 31363377

    Sample: Serum,Urine
  6. Faculty of Applied Health Sciences, Brock University (2019)
    Sclerostin Response to Exercise: Association with Bone Turnover, Oxidative Stress and Inflammation

    PMID: 99999999

    Sample: Serum
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

P***lSubmitted [ Aug 28 2018 ]

Asked: I did notice that the assay kit for P1NP (Procollagen type 1 N-terminal propeptide) reports a very low detection range: it only detects values between 15.63 - 1000 pg/mL, or otherwise could be stated as 0.015 - 1 ng/mL. However, Population normal ranges for serum P1NP are: 15 - 80 ng/mL (I\'ve attached a poster reporting those population norms; the associated reference is Jenkins et al. 2013, Bone 55:271-276). Has the company made a typing error on the assay kit insert and used incorrect units or is that the true very low limits of detection for that kit?

Reply

A

adminSubmitted [ Aug 28 2018 ]

Answered: Thanks for your question. Referred from our in-house data, the normal serum samples were diluted in 500 folds, and the serum PINP levels were 40-110ng/mL, which consistents with the reference values in publications. If the concentration of the target protein in the sample is out of the highest detection limit, it just needs to be diluted to fall in the detection range. We usually try highest sensitivity to make the kits more flexible, so the kit could meet different experiments requirements. Further inquiry, please tell us.

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