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Hydroxyl Free Radical Scavenging Capacity Assay Kit

Cat.No.:E-BC-K527-M
Detection instrument: Microplate reader(500 nm-530 nm,optimum wavelength: 510 nm)

Detection method: Colorimetric method

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48T

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Detection principle

Hydroxyl radical is a kind of reactive oxygen species, which can kill red blood cells, degrade DNA, cell membrane and polysaccharide compounds, causing damage to cell structure and function, and then leading to metabolic disorders in the body to cause diseases. The scavenging ability of hydroxyl free radical is one of the important indexes of the antioxidant ability of samples. It has been widely used in the research of antioxidant health care products and drugs.

Performance characteristics

Sample type	Serum, plasma, animal tissue
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	40 min
Precision	Average inter-assay CV: 4.600%Average intra-assay CV: 4.100%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge
Other reagents required	Absolute ethanol
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the scavenging capacity detection range (10-50%). The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Dog serum	1
Rat serum	1
Cynomolgus monkey serum	1
10% Rat spleen tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	1

Note: The diluent is double distilled water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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