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Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)

Cat.No.:E-BC-K062-M
Detection instrument: Microplate reader (550-570 nm, optimum wavelength: 558 nm)

Detection method: Colorimetric method

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Detection principle

The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.

Performance characteristics

Synonyms	HYP
Sample type	serum, animal tissue, urine
Sensitivity	0.04 μg/mL
Detection range	0.04-10 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 4.400%Average intra-assay CV: 1.200%
Other instruments required	Test tube, Glass tube, pH test strips.
Other reagents required	6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol
Storage	2-8℃
Valid period	12 months

Images

The concentration of HYP which reflects the amount of collagen was significantly increased by HDM. (**P<0.01, ***P<0.001)

L Yuan et al investigates the airway remodeling in asthma mouse by regulating SHP2/JNK/c-Jun/FGF2 through airway epithelial ITGB4 deficiency. Hydroxyproline (HYP) of mouse lung was determined using HYP colorimetric assay kit (E-BC-K062-M).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY (2022) IF: 14.29
Airway epithelial ITGB4 deficiency induces airway remodeling in a mouse model

DOI: 10.1016/j.jaci.2022.09.032

PMID: 36243221
Sample: lung

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