Current Position:Home>>Kits>>Metabolism Assays>> Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)

Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)

Cat.No.:E-BC-K062-M
Detection instrument: Microplate reader (550-570 nm, optimum wavelength: 558 nm)

Detection method: Colorimetric method

To Purchase E-BC-K062-M

Size:

96T
48T

Price: $290

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.

Performance characteristics

Synonyms	HYP
Sample type	serum, animal tissue, urine
Sensitivity	0.04 μg/mL
Detection range	0.04-10 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 4.400%Average intra-assay CV: 1.200%
Other instruments required	Test tube, Glass tube, pH test strips.
Other reagents required	6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol
Storage	2-8℃
Valid period	12 months

Images

The concentration of HYP which reflects the amount of collagen was significantly increased by HDM. (**P<0.01, ***P<0.001)

L Yuan et al investigates the airway remodeling in asthma mouse by regulating SHP2/JNK/c-Jun/FGF2 through airway epithelial ITGB4 deficiency. Hydroxyproline (HYP) of mouse lung was determined using HYP colorimetric assay kit (E-BC-K062-M).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha