Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method) (E-BC-K062-M)
For research use only.
| Detection Principle | The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm. |
| Synonyms | HYP |
| Sample Type | Serum,Plasma,Animal tissue,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (550-570 nm, optimum wavelength: 558 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | 6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.04 μg/mL |
| Detection Range | 0.04-10 μg/mL |
| Precision | inter-assay CV: 4.4% | intra-assay CV: 1.2% |
| Sample Volume | 100 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| Chicken Tendon | 20-30 |
| Fish scale | 20-30 |
| Porcine cartilage | 15-25 |
| Human Urine | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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