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Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)

  • Cat.No.:E-BC-K062-M

  • Detection instrument: Microplate reader (550-570 nm, optimum wavelength: 558 nm)

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Price: $290

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Detection principle

The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.

Performance characteristics

Synonyms HYP
Sample type serum, animal tissue, urine
Sensitivity 0.04 μg/mL
Detection range 0.04-10 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 4.400%Average intra-assay CV: 1.200%
Other instruments required Test tube, Glass tube, pH test strips.
Other reagents required 6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol
Storage 2-8℃
Valid period 12 months


The concentration of HYP which reflects the amount of collagen was significantly increased by HDM. (**P<0.01, ***P<0.001)

L Yuan et al investigates the airway remodeling in asthma mouse by regulating SHP2/JNK/c-Jun/FGF2 through airway epithelial ITGB4 deficiency. Hydroxyproline (HYP) of mouse lung was determined using HYP colorimetric assay kit (E-BC-K062-M).


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