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For research use only.

Verified Samples Verified Samples in WB: A549
Verified Samples in IHC: Rat lung, Mouse kidney
Dilution WB 1:500-1:2000,  IHC 1:100-1:200
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC
Clonality Polyclonal
Immunogen A synthetic peptide of human IL3RA (NP_002174.1).
Abbre IL3RA
Synonyms IL3RA,  CD123,  IL3R,  IL3RAY,  IL3RX,  IL3RY,  hIL-3Ra,  IL-3 Receptor alpha,  IL-3 receptor subunit alpha,  IL-3R subunit alpha,  IL-3R subunitalpha,  IL-3RA,  IL-3R-alpha,  IL-3Receptoralpha,  IL-3Rα,  IL-4Receptoralpha,  IL-5Receptoralpha,  IL-6Receptoralpha,  IL-7Receptoralpha,  Interleukin-3 receptor subunit alpha,  IL-3R&,  alpha
Swissprot
Calculated MW 34 kDa/43 kDa
Observed MW 70 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Membrane.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Immunology,  Signal transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.
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Unconjugated

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