Iron Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K139-S

      • 100Assays
      • 50Assays
      - +
      Price: $220

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(520 nm)

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Detection principle

      Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.

      Performance characteristics

      Synonyms Fe
      Sample type serum,tissue,cell culture supernatant
      Sensitivity 0.072 mg/L
      Detection range 0.072-60 mg/L
      Precision Average inter-assay CV: 2.5%Average intra-assay CV: 4.6%
      Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months
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      MSDS for ELISA ISO 9001

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