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L-Lactic Acid (LA) Colorimetric Assay Kit

  • Cat.No.:E-BC-K044-S

  • Detection instrument: Spectrophotometer(530 nm)

To Purchase E-BC-K044-S

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Price: $300

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Detection principle

Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.

Performance characteristics

Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 0.05 mmol/L
Detection range 0.05-6.0 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 1.900%Average intra-assay CV: 1.100%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months


DOX administration significantly increased hippocampal lactic acid content (*P<0.05 vs. Control group), AML co-treatment significantly lowered hippocampal lactic acid content to near control levels. AML alone treated rats showed no significant difference when compared to control group. (#P<0.05 and ###P<0.001 vs. DOX group)

A E Ali investigate the antitumor effects of AML and its ability to mitigate cancer resistance to DOX therapy. L-Lactate (L-LA) of rat hippocampal was determined using L-lactate colorimetric assay kit (E-BC-K044-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-6.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


10% Mouse muscle tissue homogenization


10% Mouse liver tissue homogenization


Rat serum


HePG2 cells homogenization (1.388 gprot/L)


HepG2 supernatant


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


  1. Biomedical Engineering Online (2021) IF: 2.819
    Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression

    DOI: 10.1186/s12938-021-00903-6

    PMID: 34217312

    Sample: Huh-8 Cells
    CircCDC6 restrains tumor growth and glycolysis energy metabolism in colorectal cancer via regulating miR-3187-3p and downstream PRKAA2

    DOI: 10.1007/s10863-022-09938-3

    PMID: 35438362

    Sample: Sw480 Cell,Hct116 Cell
  3. Molecular Medicine Reports (2021) IF: 2.952
    circ-PRKCI targets miR-1294 and miR-186-5p by downregulating FOXK1 expression to suppress glycolysis in hepatocellular carcinoma

    DOI: 10.3892/mmr.2021.12103

    Sample: Cell Sample(Hcclm3)
    Metabolic Phenotype Intricacies on Altered Glucose Metabolism of Breast Cancer Cells upon Glut-1 Inhibition and Mimic Hypoxia In Vitro

    DOI: 10.1007/s12010-023-04373-5

    Sample: Skbr-3 Breast Cancer Cell
  5. Bioengineered (2021) IF: 3.269
    KLF9 (Kruppel Like Factor 9) induced PFKFB3 (6-Phosphofructo-2-Kinase/Fructose-2, 6-Biphosphatase 3) downregulation inhibits the proliferation, metastasis and aerobic glycolysis of cutaneous squamous cell carcinoma cells

    DOI: 10.1080/21655979.2021.1980644

    PMID: 34612136

    Sample: A431 Cell
  6. ACS Omega (2022) IF: 3.512
    Specific Tumor Cell Detection by a Metabolically Targeted Aggregation-Induced Emission-Based Gold Nanoprobe

    DOI: 10.1021/acsomega.2c01494

    Sample: Mcf-7 Cell,Hepg2 Cell,Caco-2 Cell
  7. NEUROTOXICOLOGY (2021) IF: 4.294
    The diuretic amiloride attenuates doxorubicin-induced chemobrain in rats: Behavioral and mechanistic study

    DOI: 10.1016/j.neuro.2021.10.002

    PMID: 34656704

    Sample: Hippocampus
  8. iScience (2021) IF: 5.458
    Excipient of paclitaxel induces metabolic dysregulation and unfolded protein response

    DOI: 10.1016/j.isci.2021.103170

    PMID: 34646996

    Sample: Thp-1 Cell
  9. CANCER SCIENCE (2022) IF: 6.518
    circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

    DOI: 10.1111/cas.15351

    Sample: Ags Cell,Hgc-27 Cell
  10. Cell Communication and Signaling (2022) IF: 7.525
    Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth

    DOI: 10.1186/s12964-022-00882-8

    PMID: 35842652

    Sample: Sgc-996 Cell,Eh-Gb1 Cell,Gbc-Sd Cell
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  • Q&A

Verified Customer

p****uSubmitted [ Jun 19 2019 ]

  • Application:Biochemical
  • Description:This kit can be fast, reaction only takes 10 minutes, can accurately get the experimental results, less interference factors. I use this kit to detect human serum, each sample is tested three times, the difference between the results of samples is small, and all samples have a good trend. So the quality of this kit is very good.
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Verified Customer

C***ySubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:This biochemical kit is good, easy and fast. with a small sample size, but it needs fast detection
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M*****iSubmitted [ Oct 21 2022 ]

Asked: Why did my result exceed the detection range of the kit?



adminSubmitted [ Oct 21 2022 ]

Answered: If the sample content is beyond detection range, please dilute the sample before detection.


M*****iSubmitted [ Sep 13 2022 ]

Asked: What is the homogenate medium of the tissue sample?



adminSubmitted [ Sep 13 2022 ]

Answered: You can choose PBS (0.01 M, pH 7.4) or 0.9% NaCl.

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