Lactate Dehydrogenase (LDH) Activity Assay Kit (E-BC-K046-M)
For research use only.
| Detection Principle | Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value. |
| Synonyms | LDH |
| Sample Type | Serum,Plasma,Hydrothorax,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Liver And Renal Function , Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 6 U/L |
| Detection Range | 6-500 U/L |
| Precision | inter-assay CV: 2.4% | intra-assay CV: 1.8% |
| Sample Volume | 40 μL |
| Assay Time | 45 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 10 |
| Human plasma | 15-30 |
| Porcine serum | 10-20 |
| Human hydrothorax | 5-8 |
| 10% Mouse kidney tissue homogenization | 500-800 |
| 10% Mouse lung tissue homogenization | 500 |
| 10% Mouse liver tissue homogenization | 500-800 |
| HepG2 cells homogenization | 100-300 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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