Lactate Dehydrogenase (LDH) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K046-M

      Size:
      • 96T
      Qty:
      - +
      Price: $150
      Can detect 40 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(440-460 nm,optimum wavelength: 450 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.

      Performance characteristics

      Synonyms LDH
      Sample type serum,plasma,hydrothorax,tissue,cells
      Sensitivity 6 U/L
      Detection range 6-1000 U/L
      Precision Average inter-assay CV: 2.4%Average intra-assay CV: 1.8%
      Other instruments required Micropipettor, Multichannel pipette, Incubator
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 3 months

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      Citations

      1. Protective Effect of Barbaloin in a Rat Model of Myocardial Ischemia Reperfusion Injury Through the Regulation of the CNPY2‑PERK Pathway
        IF: 2.928
        Journal: International Journal of Molecular Medicine(2019)
        DOI: 10.3892/ijmm.2019.4123
        PMID: 30864682
        Products: E-BC-K046-M E-BC-K558-S 
        Sample type: Serum
        Reactivity: Rat
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      Verified Customer

      p****pSubmitted [ Jun 06 2019 ]

      • Application:Biochemical
      • Description:I used this kit to detect the content of lactate dehydrogenase in human serum and got good results. This kit is easy to operate, short detection time, with sample control to reduce interference, is a great design.
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      MSDS for ELISA ISO 9001

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