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Leucine Aminopeptidase (LAP) Activity Assay Kit

Cat.No.:E-BC-K568-M
Detection instrument: Microplate reader(405 nm)

Detection method: Colorimetric method

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96T

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Detection principle

LAP can catalyze the substrate L-leucine-4-nitroaniline to produce p-nitroaniline, which has the maximum absorption peak at the wavelength of 405 nm. The enzyme activity of LAP can be calculated by measuring the increasing OD value of the system.

Performance characteristics

Synonyms	LAP
Sample type	Serum, plasma, animal tissue
Sensitivity	5.2 U/L
Detection range	5.2-201.8 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	25 min
Precision	Average inter-assay CV: 6.400%Average intra-assay CV: 4.100%
Other instruments required	Micropipettor, Water bath, Centrifuge, Incubator, Vortex mixer
Other reagents required	Acetone
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 5.2-201.8 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Dog serum	1
Rat serum	1
Cynomolgus monkey serum	1
10% Rat spleen tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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