Lipid Peroxide (LPO) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    <
    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K176-M

      Size:
      • 48T
      • 96T
      Qty:
      - +
      Price: $280

      Detection method: Colorimetric method

      Detection instrument: Microplate reader

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      General information

      Detection significance

      Lipid peroxidation, as an indicator of oxidative stress in cell and tissue, has been identified as a kind of cellular damage. Lipid peroxide is unstable and can decompose into complex mixture including carbonyl compounds. Polyunsaturated fatty acid peroxide is decomposed into Malondialdehyde (MDA) and 4- hydroxyl olefins (HAE). Detection of LPO, MDA and HAE has been an indicator of lipid peroxidation.

      Detection principle

      With 45℃ incubation for 60 min, one molecule of LPO react with two molecule of chromogenic reagent, to produce a stable chromophore which have the maximum absorption peak at 586 nm. The content of LPO in samples can be calculated by standard curve or calculation formula.

      The key point

      1.  The EP tube needs to be sealed to avoid leakage.

      2.  The supernatant added to 96-well microplate must be clarified, otherwise centrifuge again.

      3.  Please carry out the experiment in the fume hood and wear disposable gloves, prevent the reagents splashing dashed into eyes and skin.

      Operation procedures

      Plate set up

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      A

      A

      S1

      S9

      S17

      S25

      S33

      S41

      S49

      S57

      S65

      S73

      B

      B

      B

      S2

      S10

      S18

      S26

      S34

      S42

      S50

      S58

      S66

      S74

      C

      C

      C

      S3

      S11

      S19

      S27

      S35

      S43

      S51

      S59

      S67

      S75

      D

      D

      D

      S4

      S12

      S20

      S28

      S36

      S44

      S52

      S60

      S68

      S76

      E

      E

      E

      S5

      S13

      S21

      S29

      S37

      S45

      S53

      S61

      S69

      S77

      F

      F

      F

      S6

      S14

      S22

      S30

      S38

      S46

      S54

      S62

      S70

      S78

      G

      G

      G

      S7

      S15

      S23

      S31

      S39

      S47

      S55

      S63

      S71

      S79

      H

      H

      H

      S8

      S16

      S24

      S32

      S40

      S48

      S56

      S64

      S72

      S80

                                        [Note]: A, blank wells; B-H, standard wells; S1-S80, sample wells.

      The dilution of standard curve

      Dilute 100 μmol/L standard solution with absolute ethanol to a serial concentration. The recommended dilution gradient is as follows: 80, 50, 40, 30, 20, 10, 5, 0 μmol/L.

      Operation steps

      1)    Standard well: add 200 μL of standard solution with different concentrations into the 1.5 mL EP tube.

      Sample well: add 200 μL of Sample into the 1.5 mL EP tube.  

      2)    Add 650 μL of chromogenic agent, cover the caps and mix fully.

      3)    Add 150 μL of Reagent 3, cover the caps and mix fully.

      4)    Incubate at 45 for 60 min. Cool to room temperature with running water.

      5)    Centrifuge at 1100 g for 10 min. Take 200 μL of supernatant to 96-well microplate, measure the OD values of each well at 586 nm with Microplate reader

      Operation table

       

      Standard well

      Sample well

      Sandard solution with different concentration (μL)

      200

       

      Sample (μL)

       

      200

      Cover the cap and mix fully.

      Chromogenic agent (μL)

      650

      650

      Reagent 3 (μL)

      150

      150

      Cover the cap and mix fully, incubate at 45 for 60 min. Cool to room temperature with running water. Centrifuge at 1100 g for 10 min. Take 200 μL of supernatant to 96-well microplate, measure the OD values of each well at 586 nm with Microplate reader.

      Performance characteristics

      Technical parameter

      Detection range 0.70-80 μmol/L Average inter-assay CV 3.5%
      Sensitivity 0.70 μmol/L Average intra-assay CV 3.1%
      Average recovery rate 99%
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History


        People Also Bought

        Leave Message

        *
        *
        *
        *
        *
        *
        *
        VerifyCode
        Message submitted successfully !