Lipid Peroxide (LPO) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K176

      Size:
      • 48T
      • 96T
      Qty:
      - +
      Price: $280

      Detection method: Colorimetric method

      Detection instrument: Microplate reader

      Valid period: 12 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Application

      This kit can be used to measure LPO content of all kinds of animal serum (plasma), tissue and other samples.

       

      Detection significance

      Lipid peroxidation, as an indicator of oxidative stress in cell and tissue, has been identified as a kind of cellular damage. Lipid peroxide is unstable and can decompose into complex mixture including carbonyl compounds. Polyunsaturated fatty acid peroxide is decomposed into Malondialdehyde (MDA) and 4- hydroxyl olefins (HAE). Detection of LPO, MDA and HAE has been an indicator of lipid peroxidation.

       

      Detection principle

      With 45℃ incubation for 60 min, one molecule of LPO react with two molecule of chromogenic reagent, to produce a stable chromophore which have the maximum absorption peak at 586nm. The content of LPO in samples can be calculated by standard curve or calculation formula.


      Experimental instrument

      Microplate reader (586 nm)

      Micropipettor

      Vortex mixer

      Water bath

      Low-speed centrifuge


      Sample preparation

      1. Serum/plasma: carry out the assay directly. If the concentration is beyond the linear range, then dilute the sample with saline before detection.

       

      2. Tissue: Mince the tissues to small pieces, then be weighed and homogenized in normal saline on ice, the volume of normal saline (mL): the weight of the tissue (g) =9:1. Then centrifuge at 2500 rpm for 10 min. Collect the supernatant and carry out the assay immediately. Meanwhile, determine the concentration of supernatant (E-BC-K318, E-BC-K168, E-BC-K165).


      Operation steps

         Take the 1.5 mL EP tubes (with cap), label the number, then carry out the assay according to the following table.

       

      Blank tube

      Standard tube

      Sample tube

      Absolute Ethanol (self-prepared) (μL)

      200

       

       

      10 μmol/L standard solution (μL)

       

      200

       

      Sample (μL)

       

       

      200

      Reagent 1 application solution (μL)

      650

      650

      650

      Cover the cap and mix fully.

      Reagent 2 (μL)

      150

      150

      150

      Cover the cap and mix fully, incubate at 45 for 60 min. Centrifuge at 4000 rpm for 10 min. Take 200 μL of supernatant to 96 well micro-plate, measure the OD values of each well at 586 nm.

         Note: please wear disposable gloves, and prevent the reagents splashing dashed into eyes and skin.




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