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M199 (without L-glutamine)

  • Cat.No.:EP-CM-L0222

To Purchase EP-CM-L0222

Size:
  • 500mL
Price: $20
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Background

M199 (Medium 199) originally used in nutritional studies of chicken embryo fibroblasts, it has been widely used in the culture of various animal cells, including some non-mammalian cells. M199 is especially suitable for the culture of untransformed cells, rat pancreatic epithelial cells and mouse lens tissues, also used in virology and vaccine production. Compared with other basic medium, M199 contains unique ingredients, including adenine, adenosine, hypoxanthine, thymine, and other vitamins. There are two kinds of equilibrium salt (earle's salt and hank's salt) in M199. Earle's salt is often used in CO2 environment, hank's salt is used in non-CO2 environment. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

General Information

Product Form Liquid
Concentration
pH 7.2-7.4
D-Glucose 1000mg/L
HEPES Negative
L-glutamine Negative
NaHCO3 2200mg/L
Phenol red 20mg/L
Balanced salt Earle's salt
Storage 2-8°C, Shading light
Shipping RT
Expiration date 12 months

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Notes

1. This product is for research use only.
2. This product is sterilized by 0.22 μ m filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution during the culture.

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