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Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)

Citations(16)

Cat.No.:E-BC-K028-M
Detection instrument: Microplate reader(530-540 nm,optimum wavelength: 532 nm)

Detection method: Colorimetric method

To Purchase E-BC-K028-M

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500Assays

Price: $280

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Protocols for metabolism assays

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★: Microplate is not provided in metabolism assay kits of over 500 assays.

Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

Performance characteristics

Synonyms	MDA
Sample type	Cells
Sensitivity	0.29 nmol/mL
Detection range	0.29-100 nmol/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	65 min
Precision	Average inter-assay CV: 3.500%Average intra-assay CV: 3.300%
Other instruments required	Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required	Absolute ethanol
Storage	2-8℃
Valid period	12 months

Images

The level of MDA was induced significantly in treated cells. (***P<0.001)

H Huang et al developed a nanoplatform that can induce ferroptosis for cancer therapy. Malondialdehyde (MDA) CAL-27 cell was determined using MDA colorimetric assay kit (E-BC-K028-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-100 nmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
A549 cells	1
HepG2 cells	1
293T cells	1

Note: The diluent is reagent 5.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025

Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

DOI: 10.1016/j.fct.2022.113061
PMID: 35489465	Sample: Pig Testicular Cell (St Cell)

Cancers (2023) IF: 6.575

CircZBTB46 Protects Acute Myeloid Leukemia Cells from Ferroptotic Cell Death by Upregulating SCD

DOI: 10.3390/cancers15020459
	Sample: Acute Myeloid Leukemia (Aml) Cell

Frontiers in Molecular Biosciences (2022) IF: 6.113

NPC1 Deficiency Contributes to Autophagy-Dependent Ferritinophagy in HEI-OC1 Auditory Cells

DOI: 10.3389/fmolb.2022.952608
PMID: 35936782	Sample: Hei-Oc1 Cell

BIOLOGICAL RHYTHM RESEARCH (2022) IF: 1.219

Impact of photoperiods on the specific activities of immune and antioxidant enzymes in different tissues of Dybowski’s frog (Rana dybowskii)

DOI: 10.1080/09291016.2022.2043588
	Sample: Serum,Liver,Skin

CELL STRESS & CHAPERONES (2020) IF: 2.892

Berberine attenuates mitochondrial dysfunction by inducing autophagic flux in myocardial hypoxia/reoxygenation injury

DOI: 10.1007/s12192-020-01081-5
	Sample: H9C2 Cell

DIGESTIVE DISEASES AND SCIENCES (2021) IF: 3.199

Protective Effect of miR-193a-5p and miR-320-5p on Caerulein-Induced Injury in AR42J Cells

DOI: 10.1007/s10620-020-06800-7
PMID: 33405047	Sample: Cell Sample

Bioengineered (2022) IF: 3.269

Methylprednisolone up-regulates annexin A1 (ANXA1) to inhibit the inflammation, apoptosis and oxidative stress of cigarette smoke extract (CSE)-induced bronchial epithelial cells, a chronic obstructive pulmonary disease in vitro model, through the formyl

DOI: 10.1080/21655979.2022.2031769
	Sample: Beas-2B Cells

CLINICAL CHEMISTRY AND LABORATORY MEDICINE (2021) IF: 3.694

Inflammation, vascular biology, endothelium, and oxidative stress

DOI: 10.1515/cclm-2021-5022
	Sample: Mcf-7 Cells

Annals of Translational Medicine (2022) IF: 3.932

Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

DOI: 10.21037/atm-22-483
PMID: 35280364	Sample: Myocardium

MOLECULES (2021) IF: 4.412

Insight into Seasonal Change of Phytochemicals, Antioxidant, and Anti-Aging Activities of Root Bark of Paeonia suffruticosa (Cortex Moutan) Combined with Multivariate Statistical Analysis

DOI: 10.3390/molecules26206102
PMID: 34684685	Sample: Hdf Cell

Journal of Bone Oncology (2023) IF: 4.491

Indirect bilirubin impairs invasion of osteosarcoma cells via inhibiting the PI3K/AKT/MMP-2 signaling pathway by suppressing intracellular ROS

DOI: 10.1016/j.jbo.2023.100472
	Sample: 143B Cell,Mg63 Cell

Cell Death & Disease (2020) IF: 6.304

Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload

DOI: 10.1038/s41419-020-02777-3
	Sample: Heart

ANTIOXIDANTS & REDOX SIGNALING (2022) IF: 7.468

NEAT1 regulates CaOx crystal-induced renal tubular oxidative injury via miR-130/IRF1

DOI: 10.1089/ars.2022.0008
PMID: 36242511	Sample: Hk-2 Cells

Frontiers in Immunology (2022) IF: 7.561

Identification and Validation of Ferroptosis-Related LncRNA Signatures as a Novel Prognostic Model for Colon Cancer

DOI: 10.3389/fimmu.2021.783362
	Sample: Hct116 Cell,Sw480 Cell

Acta Biomaterialia (2022) IF: 8.947

An all-in-one biomimetic iron-small interfering RNA nanoplatform induces ferroptosis for cancer therapy

DOI: 10.1016/j.actbio.2022.06.017
PMID: 35709941	Sample: Cal-27 Cell

PHARMACOLOGICAL RESEARCH (2022) IF: 10.334

Sodium-Glucose Cotransporter-2 (SGLT2) expression in diabetic and non-diabetic failing human cardiomyocytes

DOI: 10.1016/j.phrs.2022.106448
PMID: 36096423	Sample: Ac16 Cell

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Reviews/Q&A

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Reviews
Q&A

A*********oSubmitted [ Aug 17 2024 ]

Asked: Is it possible to use the Bradford method for protein quantification instead of the BCA kit?

adminSubmitted [ Aug 17 2024 ]

Answered: Yeah, for E-BC-K028-M kit, the Bradfrod method can also be used to detect protein concentration.

R****************** Submitted [ Jun 25 2024 ]

Asked: Hi, I was wondering if this kit can be used for supernatant from mouse skin? My samples are mouse skin, which I homogenise in RIPA and centrifuge. The resulting supernatant is what I will use for the assay.

adminSubmitted [ Jun 25 2024 ]

Answered: 1. E-BC-K028-M is suitable for the detection of cell samples. If the MDA content of tissue samples is detected, it is recommended that you take a look at the E-BC-K025-M kit. 2. Since the detection principle of E-BC-K025-M kit is chemical reaction, the composition of RIPA cracking solution is relatively complex, which may have an impact on the reaction system. It is not recommended to treat samples with RIPA lysate. 3. If testing tissue samples, sample preparation is recommended in accordance with the manual. For skin tissue, it is recommended to extend the homogenate time until the tissue is fully broken.

E*** Submitted [ Apr 27 2024 ]

Asked: Hi, I\'m using HaCaT cells. Firstly, may I know if I can use RIPA buffer instead of the reagent 5 (extraction solution) in the E-BC-K028-M kit? Secondly, does using Trypsin for cell detachment is contraindicated even if I rinse twice with PBS?

adminSubmitted [ Apr 27 2024 ]

Answered: Dear Elly, Regards to your questions, please refer to the feedbacks below: 1. Since E-BC-K028-M kit is developed on the principle of chemical reaction, the complex composition of RIPA buffer may have an impact on the reaction system. It is not recommended to replace reagent 5 with RIPA buffer to treat cells. 2. If it's to detect the adherent cells, the cells can be digested with trypsin or scraped off with cell scraper. If it's digested with trypsin, it needs to wash with PBS for 2~3 times. After that it would not affect the result. Hope it helps Best Regards Lizzy

M***********uSubmitted [ Sep 25 2023 ]

Asked: Which of your MDA reagents would you suggest for Drosophila homogenate sample?

adminSubmitted [ Sep 25 2023 ]

Answered: E-BC-K025-M/E-BC-K025-S

N****aSubmitted [ Jun 15 2023 ]

Asked: Hi, can I know if reagent 5 is the extraction solution? Meaning that we don’t have to use other homogenization medium such as Triton-X? I tried few times and the standard is the only group that gives me colour.

adminSubmitted [ Jun 15 2023 ]

Answered: Hi Nabila. Yes. We suggest to increase the concentration of cell homogenization. And try to read the OD value, the color might be quite light.

錯*********nSubmitted [ Jun 12 2023 ]

Asked: Can I use E-BC-K028-M kit with NRK-52e cells?

adminSubmitted [ Jun 12 2023 ]

Answered: Hello HuieLian.Thanks for your support.This kit is suitable for NRK-52e cells samples.

錯*********nSubmitted [ Jun 12 2023 ]

Asked: Can I use E-BC-K028-M kit with NRK-52e cells?

adminSubmitted [ Jun 12 2023 ]

Answered: Hello HuieLian.Thanks for your support.This kit is suitable for NRK-52e cells samples.

F***hSubmitted [ Jun 16 2022 ]

Asked: Hi, can I know if reagent 5 is the extraction solution? Meaning that we don’t have to use other homogenization medium such as Triton-X?

adminSubmitted [ Jun 16 2022 ]

Answered:

L********gSubmitted [ Mar 31 2022 ]

Asked: When using E-BC-K028-M kit, what is the minimum cells required in one test?

adminSubmitted [ Mar 31 2022 ]

Answered: Dear Linus,the minimum cells required in one test are 3*10^6. best regards,

M*****aSubmitted [ Jan 28 2022 ]

Asked: Since I have to determine the protein concentration using Bradford, can I use the protein extract obtained after reagent 5 addition for a Western Blot run? or are there some buffers in reagent 5 that can potentially interfer with the gel run?

adminSubmitted [ Jan 28 2022 ]

Answered: Dear Martina Thanks for your message. For the inquired item reagent 5 in kit E-BC-K028-M Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples) is only suitable for the kit E-BC-K028-M, while if you are searching for a Lysis Buffer for WB run, we advise you can try another item E-BC-R327 RIPA Lysis Buffer https://www.elabscience.com/search-keywords=e-bc-r327.html Hope to support you. Best regards

D********tSubmitted [ Feb 02 2021 ]

Asked: Can I use E-BC-K028-M kit with serum and tissue homogenate samples?

adminSubmitted [ Feb 02 2021 ]

Answered: You can choose the E-BC-K025-M kit.

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