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Malondialdehyde (MDA) Colorimetric Assay Kit (Plant Samples)

Cat.No.:E-BC-K027-S
Detection instrument: Spectrophotometer(532 nm)

Detection method: Colorimetric method

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Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

Performance characteristics

Synonyms	MDA
Sample type	Plant tissue
Sensitivity	0.17 nmol/mL
Detection range	0.17-120 nmol/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	90 min
Precision	Average inter-assay CV: 6.100%Average intra-assay CV: 2.200%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Other reagents required	Absolute ethanol
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.17-120 nmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Epipremnum aureum tissue homogenate	1
10% Green pepper tissue homogenate	1
10% Eggplant tissue homogenate	1
10% Bitter gourd tissue homogenate	1

Note: The diluent is reagent 5 working solution.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Annals of Translational Medicine (2022) IF: 3.932
Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

DOI: 10.21037/atm-22-483

PMID: 35280364
Sample: myocardium
BIOLOGICAL RHYTHM RESEARCH (2022) IF: 1.219
Impact of photoperiods on the specific activities of immune and antioxidant enzymes in different tissues of Dybowski’s frog (Rana dybowskii)

DOI: 10.1080/09291016.2022.2043588

Sample: serum,liver,skin

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