Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method) (E-BC-K025-M)
For research use only.
| Detection Principle | MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm. |
| Synonyms | MDA |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-540 nm, optimum wavelength: 532 nm) |
| Research Area | Oxidative Stress , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.13 μmol/L |
| Detection Range | 2.92-40 μmol/L |
| Precision | inter-assay CV: 7.2% | intra-assay CV: 4.1% |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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