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Maltase Activity Assay Kit

Cat.No.:E-BC-K041-M
Detection instrument: Microplate reader(500 nm-520 nm,optimum wavelength: 505 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Maltase catalyze the corresponding substrate to produce monosaccharide. Monosaccharide produce hydrogen peroxide under the action of oxidase. Hydrogen peroxide react with chromogenic agent to form red product. The activity of maltase can be calculated by detection the optical density with spectrophotometer at 505 nm.

Performance characteristics

Sample type	Animal tissue
Sensitivity	6.32 U/mL
Detection range	6.32-750 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 5.700%Average intra-assay CV: 2.700%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (6.32-750 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat heart tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Mouse intestinal tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Mouse brain tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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