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Mouse IL-4(Interleukin 4) ELISA Kit

Citations(24)Uniprot : P07750

  • Cat.No.:E-EL-M0043

  • Reactivity: Mouse
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-4 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 97.60 252.20 947.10 99.90 274.50 966.50
Standard deviation 6.50 11.30 30.30 6.40 14.00 36.70
CV (%) 6.66 4.48 3.20 6.41 5.10 3.80

Recovery

The recovery of Mouse IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 91
EDTA plasma (n=8) 95-111 101
Cell culture media (n=8) 92-108 98

Linearity

Samples were spiked with high concentrations of Mouse IL-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P07750
SynonymsIL4, BCGF-1, BCGF1, BSF-1, BSF1
Research AreaCancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Chemosphere (2019) IF: 5.108
    Maternal 1-nitropyrene exposure during pregnancy increases susceptibility of allergic asthma in adolescent offspring

    DOI: 10.1016/j.chemosphere.2019.125356

    PMID: 31743867

    		 Sample: Tissue homogenate
  2. Oncotarget (2017) IF: 5.168
    Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis.

    DOI: 10.18632/oncotarget.22132

    PMID: 29228739

    		 Sample: Tissue homogenate
  3. Journal of Cellular and Molecular Medicine (2020) IF: 4.486
    Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes-associated protein

    DOI: 10.1111/jcmm.15485

    PMID: 32542982

    		 Sample: Tissue homogenate
  4. ACS Chemical Neuroscience (2020) IF: 4.486
    MG53 Protects hUC-MSCs against Inflammatory Damage and Synergistically Enhances Their Efficacy in Neuroinflammation Injured Brain through Inhibiting NLRP3/Caspase-1/IL-1β Axis

    DOI: 10.1021/acschemneuro.0c00268

    PMID: 32786312

    		 Sample: Serum
  5. Journal of Agricultural and Food Chemistry (2020) IF: 4.192
    Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

    DOI: 10.1021/acs.jafc.0c00461

    PMID: 32182051

    		 Sample: Serum,Cell culture supernatant
  6. Microbial Cell Factories (2020) IF: 4.187
    A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

    DOI: 10.1186/s12934-020-01308-1

    PMID: 32075660

    		 Sample: Cell culture supernatant
  7. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    		 Sample: Serum,Cell culture supernatant
  8. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway

    DOI: 10.1016/j.ecoenv.2020.110491

    PMID: 32213367

    		 Sample: Cell culture supernatant
  9. Scientific Reports (2017) IF: 4.259
    Rheb1 Deletion In Myeloid Cells Aggravates OVA-Induced Allergic Inflammation In Mice.

    DOI: 10.1038/srep42655

    PMID: 28225024

    		 Sample: Bronchoalveolar Lavage Fluid,Serum
  10. Frontiers in Microbiology (2017) IF: 4.076
    Oral Immunization With Recombinant Lactobacillus Acidophilus Expressing espA-Tir-M Confers Protection Against Enterohemorrhagic Escherichia Coli O157: H7 Challenge In Mice.

    DOI: 10.3389/fmicb.2017.00417

    PMID: 28360900

    		 Sample: Serum
  11. Life Sciences (2020) IF: 3.647
    DHCR24 overexpression modulates microglia polarization and inflammatory response via Akt/GSK3β signaling in Aβ 25-35 treated BV-2 cells

    DOI: 10.1016/j.lfs.2020.118470

    PMID: 32950573

    		 Sample: Cell sample
  12. International Immunopharmacology (2020) IF: 3.943
    Myricitrin pretreatment ameliorates mouse liver ischemia reperfusion injury

    DOI: 10.1016/j.intimp.2020.107005

    PMID: 33045574

    		 Sample: Serum
  13. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Effects of iron chelating agent on Schistosoma mansoni infected murine model

    DOI: 10.1016/j.biopha.2018.10.036

    PMID: 30391706

    		 Sample: Serum,Tissue homogenate
  14. Annals of Allergy Asthma & Immunology (2013) IF: 3.728
    Therapeutic Effects Of Anti-B7-H3 Antibody In An Ovalbumin-Induced Mouse Asthma Model.

    DOI: 10.1016/j.anai.2013.06.030

    PMID: 24054363

    		 Sample: Bronchoalveolar Lavage Fluid
  15. Microbial Pathogenesis (2020) IF: 2.914
    Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection

    DOI: 10.1016/j.micpath.2020.104441

    PMID: 32781100

    		 Sample: Serum,Cell culture supernatant
  16. Microbial Pathogenesis (2020) IF: 2.914
    Exposure time determines the protective effect of Trichinella spiralis on experimental colitis

    DOI: 10.1016/j.micpath.2020.104263

    PMID: 32442663

    		 Sample: Serum
  17. Biological research (2019) IF: 2.357
    Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice

    DOI: 10.1186/s40659-019-0216-9

    PMID: 30823938

    		 Sample: Plasma
  18. Microbial Pathogenesis (2017) IF: 2.009
    Intranasal Immunization With Novel EspA-Tir-M Fusion Protein Induces Protective Immunity Against Enterohemorrhagic Escherichia coli O157: H7 Challenge In Mice.

    DOI: 10.1016/j.micpath.2017.01.062

    PMID: 28163157

    		 Sample: Serum
  19. Gene (2016) IF: 2.415
    Immunogenicity Of The Recombinant HxuCBA Proteins Encoded By HxuCBA Gene Cluster Of Haemophilus Parasuis In Mice.

    DOI: 10.1016/j.gene.2016.07.001

    PMID: 27378742

    		 Sample: Serum
  20. Parasitology Research (2016) IF: 2.329
    Immunopathological Assessments Of Human Blastocystis Spp. In Experimentally Infected Immunocompetent And Immunosuppresed Mice.

    DOI: 10.1007/s00436-016-4951-3

    PMID: 26860840

    		 Sample: Serum,Tissue homogenate
  21. Journal of Virological Methods (2020) IF: 1.786
    Colonisation of mice and pigs by a chimeric porcine circovirus 1-2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines

    DOI: 10.1016/j.jviromet.2020.113905

    PMID: 32502500

    		 Sample: Cell culture supernatant
  22. The FASEB Journal (2020)
    CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR

    PMID: 32862457

    		 Sample: Cell culture supernatant,Tissue homogenate
  23. Functional Foods in Health and Disease (2018)
    Immunological effects of Kefir produced from Kefir grains versus starter cultures when fed to mice

    DOI: 10.31989/ffhd.v8i8.533

    PMID: 99999999

    		 Sample: Tissue homogenate
  24. Journal of Huazhong University Of Science And Technology-Medical Sciences (2016) IF: 0.964
    Peroxisome Proliferator-Activated Receptor-Γ Agonist Pioglitazone Fails To Attenuate Renal Fibrosis Caused By Unilateral Ureteral Obstruction In Mice.

    DOI: 10.1007/s11596-016-1539-1

    PMID: 26838738

    		 Sample: Tissue homogenate
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