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Mouse IL-4(Interleukin 4) ELISA Kit

Citations(65)Uniprot : P07750

  • Cat.No.:E-EL-M0043

  • Reactivity: Mouse
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 12 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 12 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-4 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 97.60 252.20 947.10 99.90 274.50 966.50
Standard deviation 6.50 11.30 30.30 6.40 14.00 36.70
CV (%) 6.66 4.48 3.20 6.41 5.10 3.80

Recovery

The recovery of Mouse IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 91
EDTA plasma (n=8) 95-111 101
Cell culture media (n=8) 92-108 98

Linearity

Samples were spiked with high concentrations of Mouse IL-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P07750
SynonymsIL4, BCGF-1, BCGF1, BSF-1, BSF1
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Cell Death Discovery (2022) IF: 5.241
    Immunohistochemistry Troubleshooting Tips

    DOI: 10.1038/s41420-022-00960-3

    PMID: 35383145

    		 Sample: Serum
  2. Oncotarget (2017) IF: 5.168
    Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis

    DOI: 10.18632/oncotarget.22132
    Sample: Tissue Homogenate
  3. Frontiers in Microbiology (2022) IF: 6.064
    Recombinant Listeria ivanovii strain expressing listeriolysin O in place of ivanolysin O might be a potential antigen carrier for vaccine construction

    DOI: 10.3389/fmicb.2022.962326

    PMID: 35935244

    		 Sample: Splenocytes
  4. JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
    Immunomodulatory effects and mechanisms of Tiepishihu Xiyangshen granules on cyclophosphamide induced immuno-suppression via TLR4/MAPKs and PI3K/AKT/FOXO3a signal pathways

    DOI: 10.1016/j.jep.2023.116192
    Sample: Serum
  5. Clinical and Translational Allergy (2022) IF: 5.871
    MiR-29b regulates Th2 cell differentiation in asthma by targeting inducible B7-H3 and STAT3

    DOI: 10.1002/clt2.12114

    PMID: 35079347

    		 Sample: Bronchoalveolar Lavage Fluid (Balf),Lung Tissue
  6. Journal of Huazhong University of Science and Technology-Medical Sciences (2016) IF: 0.838
    Peroxisome proliferator-activated receptor-γ agonist pioglitazone fails to attenuate renal fibrosis caused by unilateral ureteral obstruction in mice

    DOI: 10.1007/s11596-016-1539-1
    Sample: Tissue Homogenate
  7. JOURNAL OF VETERINARY MEDICAL SCIENCE (2022) IF: 1.105
    Immunogenicity and protection against Glaesserella parasuis serotype 13 infection after vaccination with recombinant protein LolA in mice

    DOI: 10.1292/jvms.22-0203

    PMID: 36216558

    		 Sample: Serum
  8. JOURNAL OF VETERINARY MEDICAL SCIENCE (2021) IF: 1.267
    Application of mouse model for evaluation of recombinant LpxC and GmhA as novel antigenic vaccine candidates of Glaesserella parasuis serotype 13

    DOI: 10.1292/jvms.21-0298

    PMID: 34393140

    		 Sample: Serum
  9. ACTA PARASITOLOGICA (2021) IF: 1.44
    Evaluation of Allicin Against Alveolar Echinococcosis In Vitro and in a Mouse Model

    DOI: 10.1007/s11686-021-00434-z

    PMID: 34143400

    		 Sample: Serum
  10. PARASITOLOGY RESEARCH (2020) IF: 1.641
    Effects of programmed cell death protein 10 on fecundity in Schistosoma japonicum

    DOI: 10.1007/s00436-020-06635-1
    Sample: Serum
  11. JOURNAL OF VIROLOGICAL METHODS (2020) IF: 1.786
    Colonisation of mice and pigs by a chimeric porcine circovirus 1–2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines

    DOI: 10.1016/j.jviromet.2020.113905
    Sample: Cell Culture Supernatant
  12. MICROBIAL PATHOGENESIS (2017) IF: 2.009
    Intranasal immunization with novel EspA-Tir-M fusion protein induces protective immunity against enterohemorrhagic Escherichia coli O157:H7 challenge in mice

    DOI: 10.1016/j.micpath.2017.01.062
    Sample: Serum
  13. PARASITOLOGY RESEARCH (2016) IF: 2.027
    spp. in experimentally infected immunocompetent and immunosuppresed mice

    DOI: 10.1007/s00436-016-4951-3
    Sample: Serum,Tissue Homogenate
  14. BIOLOGICAL RESEARCH (2019) IF: 2.482
    Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice

    DOI: 10.1186/s40659-019-0216-9

    PMID: 30823938

    		 Sample: Plasma
  15. MICROBIAL PATHOGENESIS (2019) IF: 2.581
    Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

    DOI: 10.1016/j.micpath.2019.103557
    Sample: Serum,Small Intestinal Tissues
  16. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway

    DOI: 10.1155/2022/7421265

    PMID: 35178108

    		 Sample: Serum
  17. BMC Veterinary Research (2021) IF: 2.741
    The construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice

    DOI: 10.1186/s12917-021-02885-y

    PMID: 33947419

    		 Sample: Serum
  18. PLoS One (2018) IF: 2.766
    A trivalent Apx-fusion protein delivered by E. coli outer membrane vesicles induce protection against Actinobacillus pleuropneumoniae of serotype 1 and 7 challenge in a murine model

    DOI: 10.1371/journal.pone.0191286
    Sample: Serum
  19. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection

    DOI: 10.1016/j.micpath.2020.104441

    PMID: 32781100

    		 Sample: Serum,Cell Culture Supernatant
  20. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Exposure time determines the protective effect of Trichinella spiralis on experimental colitis

    DOI: 10.1016/j.micpath.2020.104263

    PMID: 32442663

    		 Sample: Serum
  21. Molecular Medicine Reports (2019) IF: 2.952
    BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice

    DOI: 10.3892/mmr.2019.10780
    Sample: Bmdcs
  22. ONCOLOGY REPORTS (2018) IF: 2.976
    Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model

    DOI: 10.3892/or.2017.6094
    Sample: Serum
  23. PeerJ (2021) IF: 2.984
    Glucosylceramide synthase regulates hepatocyte repair after concanavalin A-induced immune-mediated liver injury

    DOI: 10.7717/peerj.12138

    PMID: 34611503

    		 Sample: Serum
  24. Food & Function (2018) IF: 3.289
    Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model

    DOI: 10.1039/C7FO01161J
    Sample: Serum
  25. VETERINARY MICROBIOLOGY (2021) IF: 3.293
    Molecular and functional characterization of HtrA protein in Actinobacillus pleuropneumoniae

    DOI: 10.1016/j.vetmic.2021.109058

    PMID: 33862332

    		 Sample: Serum
  26. PHARMACOLOGY (2023) IF: 3.429
    DPP4 Inhibitor Sitagliptin Reduces Inflammatory Responses and Mast Cell Activation in Allergic Rhinitis

    DOI: 10.1159/000528634
    Sample: Serum，Nasal Lavage Fluid
  27. ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2013) IF: 3.449
    Therapeutic effects of anti–B7-H3 antibody in an ovalbumin-induced mouse asthma model

    DOI: 10.1016/j.anai.2013.06.030
    Sample: Bronchoalveolar Lavage Fluid
  28. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Effects of iron chelating agent on Schistosoma mansoni infected murine model

    DOI: 10.1016/j.biopha.2018.10.036

    PMID: 30391706

    		 Sample: Serum,Tissue Homogenate
  29. LIFE SCIENCES (2020) IF: 3.647
    DHCR24 overexpression modulates microglia polarization and inflammatory response via Akt/GSK3β signaling in Aβ25–35 treated BV-2 cells

    DOI: 10.1016/j.lfs.2020.118470
    Sample: Cell Sample
  30. GENE (2016) IF: 3.688
    Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice

    DOI: 10.1016/j.gene.2016.07.001
    Sample: Serum
  31. Parasites & Vectors (2021) IF: 3.876
    In vivo and in vitro efficacy of crocin against Echinococcus multilocularis

    DOI: 10.1186/s13071-021-04866-4

    PMID: 34256821

    		 Sample: Serum
  32. Parasites & Vectors (2021) IF: 3.876
    In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis

    DOI: 10.1186/s13071-021-04952-7

    PMID: 34488852

    		 Sample: Serum
  33. JOURNAL OF CELLULAR PHYSIOLOGY (2018) IF: 3.923
    Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post‐traumatic osteoarthritis

    DOI: 10.1002/jcp.26460
    Sample: Serum,Preosteoblasts
  34. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Myricitrin pretreatment ameliorates mouse liver ischemia reperfusion injury

    DOI: 10.1016/j.intimp.2020.107005

    PMID: 33045574

    		 Sample: Serum
  35. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Borneol inhibits CD4 + T cells proliferation by down-regulating miR-26a and miR-142-3p to attenuate asthma

    DOI: 10.1016/j.intimp.2020.107223
    Sample: Bronchoalveolar Lavage Fluid
  36. JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020) IF: 4.018
    Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice

    DOI: 10.1016/j.jri.2020.103240

    PMID: 33166807

    		 Sample: Serum
  37. Frontiers in Microbiology (2017) IF: 4.076
    Oral Immunization with Recombinant Lactobacillus acidophilus Expressing espA-Tir-M Confers Protection against Enterohemorrhagic Escherichia coli O157:H7 Challenge in Mice

    DOI: 10.3389/fmicb.2017.00417
    Sample: Serum
  38. INFLAMMATION (2022) IF: 4.092
    Pre-exposure to Aerosolized Polyvalent Bacterial Lysates Protects Against Bleomycin-Induced Pulmonary Fibrosis in Mice

    DOI: 10.1007/s10753-022-01653-w

    PMID: 35249190

    		 Sample: Bronchoalveolar Lavage Fluid (Balf)
  39. Microbial Cell Factories (2020) IF: 4.187
    A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

    DOI: 10.1186/s12934-020-01308-1

    PMID: 32075660

    		 Sample: Cell Culture Supernatant
  40. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020) IF: 4.192
    Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

    DOI: 10.1021/acs.jafc.0c00461

    PMID: 32182051

    		 Sample: Serum,Cell Culture Supernatant
  41. Scientific Reports (2017) IF: 4.259
    Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice

    DOI: 10.1038/srep42655
    Sample: Bronchoalveolar Lavage Fluid,Serum
  42. MOLECULES (2022) IF: 4.412
    Plant-Derived Molecule 4-Methylumbelliferone Suppresses FcεRI-Mediated Mast Cell Activation and Allergic Inflammation

    DOI: 10.3390/molecules27051577
    Sample: Blood
  43. NEUROCHEMICAL RESEARCH (2022) IF: 4.414
    The protective effect of Palmatine on depressive like behavior by modulating microglia polarization in LPS-induced mice

    DOI: 10.1007/s11064-022-03672-3

    PMID: 35917005

    		 Sample: Hippocampus,Bv2 Cell
  44. ACS Chemical Neuroscience (2020) IF: 4.486
    MG53 Protects hUC-MSCs against Inflammatory Damage and Synergistically Enhances Their Efficacy in Neuroinflammation Injured Brain through Inhibiting NLRP3/Caspase-1/IL-1β Axis

    DOI: 10.1021/acschemneuro.0c00268

    PMID: 32786312

    		 Sample: Serum
  45. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes‐associated protein

    DOI: 10.1111/jcmm.15485
    Sample: Tissue Homogenate
  46. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    		 Sample: Serum,Cell Culture Supernatant
  47. Frontiers in Immunology (2019) IF: 4.716
    Severe Fever With Thrombocytopenia Syndrome Virus-Induced Macrophage Differentiation Is Regulated by miR-146

    DOI: 10.3389/fimmu.2019.01095
    Sample: Serum
  48. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872
    Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway

    DOI: 10.1016/j.ecoenv.2020.110491

    PMID: 32213367

    		 Sample: Cell Culture Supernatant
  49. MOLECULES (2022) IF: 4.927
    Ethyl Acetate Extract from Artemisia argyi Prevents Liver Damage in ConA-Induced Immunological Liver Injury Mice via Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways

    DOI: 10.3390/molecules27227883
    Sample: Hepatic
  50. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    The immunomodulatory effects of ginsenoside derivative Rh2-O on splenic lymphocytes in H22 tumor-bearing mice is partially mediated by TLR4

    DOI: 10.1016/j.intimp.2021.108316

    PMID: 34768129

    		 Sample: Splenic Lymphocytes
  51. FASEB JOURNAL (2020) IF: 4.966
    CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR
    Sample: Cell Culture Supernatant,Tissue Homogenate,Serum
  52. Scientific Reports (2022) IF: 4.996
    Human umbilical cord mesenchymal stem cells derived extracellular vesicles regulate acquired immune response of lupus mouse in vitro

    DOI: 10.1038/s41598-022-17331-8

    PMID: 35908050

    		 Sample: Hucmsc
  53. CHEMOSPHERE (2019) IF: 5.108
    Maternal 1-nitropyrene exposure during pregnancy increases susceptibility of allergic asthma in adolescent offspring

    DOI: 10.1016/j.chemosphere.2019.125356

    PMID: 31743867

    		 Sample: Tissue Homogenate
  54. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021) IF: 5.279
    Reducing the Allergenicity of α-Lactalbumin after Lipid Peroxidation

    DOI: 10.1021/acs.jafc.1c00559

    PMID: 33974424

    		 Sample: Cell Culture Supernatant
  55. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Targeting PERK mediated endoplasmic reticulum stress attenuates neuroinflammation and alleviates lipopolysaccharide-induced depressive-like behavior in male mice

    DOI: 10.1016/j.intimp.2022.109092

    PMID: 35940075

    		 Sample: Plasma
  56. Frontiers in Aging Neuroscience (2022) IF: 5.75
    Agomelatine Prevents Amyloid Plaque Deposition, Tau Phosphorylation, and Neuroinflammation in APP/PS1 Mice

    DOI: 10.3389/fnagi.2021.766410
    Sample: Hippocampus Tissue
  57. Antioxidants (2021) IF: 6.313
    Aronia melanocarpa Extract Fermented by Lactobacillus plantarum EJ2014 Modulates Immune Response in Mice

    DOI: 10.3390/antiox10081276

    PMID: 34439524

    		 Sample: Plasma
  58. Food & Function (2022) IF: 6.317
    Peanut allergen induces more serious allergic reactions than other allergens involving MAPK signaling pathways

    DOI: 10.1039/D2FO00777K

    PMID: 35920097

    		 Sample: Serum
  59. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2021) IF: 6.514
    Design, synthesis, and biological evaluation of carbamate derivatives of N-salicyloyl tryptamine as multifunctional agents for the treatment of Alzheimer's disease

    DOI: 10.1016/j.ejmech.2021.114044

    PMID: 34923430

    		 Sample: Bv2 Cell
  60. IMMUNOLOGY (2021) IF: 7.397
    PTBP1 is necessary for dendritic cells to regulate T-cell homeostasis and antitumour immunity

    DOI: 10.1111/imm.13304

    PMID: 33421118

    		 Sample: Bronchoalveolar Lavage Fluid
  61. FOOD CHEMISTRY (2021) IF: 7.514
    A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols

    DOI: 10.1016/j.foodchem.2021.131589
    Sample: Spleen Cells
  62. Antioxidants (2022) IF: 7.675
    Genipin Attenuates Diabetic Cognitive Impairment by Reducing Lipid Accumulation and Promoting Mitochondrial Fusion via FABP4/Mfn1 Signaling in Microglia

    DOI: 10.3390/antiox12010074
    Sample: Hippocampal Tissues
  63. CHEMOSPHERE (2022) IF: 8.943
    Oral administration of Bacillus cereus GW-01 alleviates the accumulation and detrimental effects of β-cypermethrin in mice

    DOI: 10.1016/j.chemosphere.2022.137333
    Sample: Serum
  64. Acta Biomaterialia (2022) IF: 8.947
    ROS-responsive polymer nanoparticles with enhanced loading of dexamethasone effectively modulate the lung injury microenvironment

    DOI: 10.1016/j.actbio.2022.06.024

    PMID: 35724918

    		 Sample: Raw264.7 Cell
  65. ENVIRONMENTAL POLLUTION (2022) IF: 9.988
    Juvenile arsenic exposure aggravates goblet cell hyperplasia and airway mucus secretion in ovalbumin-sensitized mice

    DOI: 10.1016/j.envpol.2022.120462

    PMID: 36270563

    		 Sample: Lung Tissue
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