Mouse IL-6(Interleukin 6) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-M0044

      Synonyms:IL6, BSF2, HGF, HSF, IFNB2, CDF

      • 96T
      • 24T
      - +
      Price: $495

      Reactivity: Mouse

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Mouse IL-6 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-6. You can calculate the concentration of Mouse IL-6 in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Mouse
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids


      This kit recognizes Mouse IL-6 in samples. No significant cross-reactivity or interference between Mouse IL-6 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      O.D Average Corrected
      2000 2.471
      2.487 2.427
      1000 1.682
      1.701 1.641
      500 0.945
      0.93 0.87
      250 0.421
      0.431 0.371
      125 0.267
      0.255 0.195
      62.5 0.17
      0.162 0.102
      31.25 0.109
      0.112 0.052
      0 0.05
      0.06 --


      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-6 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-6 were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 99.40 174.50 952.80 105.30 162.70 1040.70
      Standard deviation 6.40 7.70 50.50 6.50 8.10 38.50
      C V (%) 6.44 4.41 5.30 6.17 4.98 3.70


      The recovery of Mouse IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 86-100 93
      EDTA plasma (n=5) 88-101 94
      Cell culture media (n=5) 89-104 95


      Samples were spiked with high concentrations of Mouse IL-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 91-106 91-103 98-111
      Average (%) 98 98 104
      1:4 Range (%) 94-109 88-103 98-110
      Average (%) 100 94 104
      1:8 Range (%) 94-108 87-98 95-106
      Average (%) 101 93 100
      1:16 Range (%) 97-113 84-97 97-109
      Average (%) 105 91 103

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results


      1. Publication: Cheng J, Zhang L, Dai W, et al. Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B[J]. Biochemical & Biophysical Research Communications, 2015, 458(1): 140-147.
        Species: Mouse
        Sample Type: Tissue homogenates
      2. Publication: Chen L, Shen X, Chen G, et al. A Comparative Study Of The Effects Upon LPS Induced Macrophage RAW264.7 Inflammation In Vitro Of The Lipids Of Hippocampus Trimaculatus Leach[J]. Journal of Oleo Science, 2015, 64(12): 1273-1281.
        Species: Mouse
        Sample Type: Culture supernatant
      3. Publication: Yan Q, Xiang Y, Shuyu W, et al. PGC-1α Silencing Compounds The Perturbation Of Mitochondrial Function Caused By Mutant SOD1 In Skeletal Muscle Of ALS Mouse Model[J]. Frontiers in Aging Neuroscience, 2015.
        Species: Mouse
        Sample Type: Serum
      4. Publication: Wang J, Ge B, Du C, et al. Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro[J]. International Journal of Clinical and Experimental Medicine, 2015, 8(11): 20380-20390.
        Species: Mouse
        Sample Type: Culture supernatant
      5. Publication: Hao S, Li H, Sun X, et al. An Unusual Case Of Iron Deficiency Anemia Is Associated With Extremely Low Level Of Transferrin Receptor[J]. International Journal of Clinical and Experimental Pathology, 2015, 8(7): 8613-8618.
        Species: Mouse
        Sample Type: Serum
      6. Publication: Hamuti A, Li J Y, Zhou F F,et al. Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells[J]. Molecules, 2017.
        Species: Mouse
        Sample Type: Culture supernatant
      7. Publication: Wan G, Lingao R, Yu Y, et al. Effects Of Silver Nanoparticles In Combination With Antibiotics On The Resistant Bacteria Acinetobacter Baumannii[J]. International Journal of Nanomedicine, 2016, 11: 3789-3800.
        Species: Mouse
        Sample Type: Plasma
      8. Publication: Wang J, Ge B, Li Z, et al. Structural Analysis And Immunoregulation Activity Comparison Of Five Polysaccharides From Angelica Sinensis[J]. Carbohydrate Polymers, 2016, 140: 6-12.
        Species: Mouse
        Sample Type: Culture supernatant
      9. Publication: Hu M, Luo Q, Alitongbieke G, et al. Celastrol-Induced Nur77 Interaction With TRAF2 Alleviates Inflammation By Promoting Mitochondrial Ubiquitination And Autophagy[J]. Molecular Cell, 2017, 66(1): 141-153.
        Species: mouse
        Sample Type: Serum
      10. Publication: Aipire A, Li J, Yuan P, et al. Glycyrrhiza Uralensis Water Extract Enhances Dendritic Cell Maturation And Antitumor Efficacy Of HPV Dendritic Cell-Based Vaccine[J]. Scientific Reports, 2017.
        Species: Mouse
        Sample Type: Culture supernatant
      11. Publication: Li H D, Chen X, Yang Y, et al. Wogonin Attenuates Inflammation By Activating PPAR-γ In Alcoholic Liver Disease[J]. International Immunopharmacology, 2017, 50: 95-106.
        Species: Mouse
        Sample Type: Culture supernatant and Serum
      12. Publication: Zhang M, Zhao H T, Cai J, et al. Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet[J]. PloS one, 2017.
        Species: Mouse
        Sample Type: Serum
      13. Publication: Valdor R, García-Bernal D, Bueno C, et al. Glioblastoma Progression Is Assisted By Induction Of Immunosuppressive Function Of Pericytes Through Interaction With Tumor Cells[J]. Oncotarget, 2017, 8(40): 68614-68626.
        Species: Mouse
        Sample Type: Culture supernatant
      14. Publication: Chen L, Zhou Z Y, Yang Y, et al. Therapeutic Effect Of imiquimod On Dextran Sulfate Sodium-induced Ulcerative Colitis In Mice[J]. Plos One, 2017.
        Species: Mouse
        Sample Type: Serum
      15. Publication: Cai Y, Sun W, Zhang X X, et al. Osthole Prevents Acetaminophen-Induced Liver Injury In Mice[J]. Acta Pharmacologica Sinica, 2017.
        Species: Mouse, Universal
        Sample Type: Tissue homogenate
      16. Publication: Wang F, Peng P L, Lin X, et al. Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis[J]. Oncotarget, 2017, 8(58): 98945-98952.
        Species: Mouse
        Sample Type: Tissue homogenate
      17. Publication: Wang Y M, Liu Z Y, Ai Y H, et al. Blocking The CD38/cADPR Pathway Plays A Double-edged Role In LPS Stimulated Microglia[J]. Neuroscience, 2017, 361: 34-42.
        Species: Mouse
        Sample Type: Culture supernatant
      18. Publication: Wu D D, Gao B, Li M L, et al. Hydrogen Sulfide Mitigates Kidney Injury In High Fat Diet-Induced Obese Mice[J]. Oxidative Medicine And Cellular Longevity, 2016.
        Species: Mouse
        Sample Type: Plasma
      19. Publication: Rao S S, Hu Y, Xie P L, et al. Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines[J]. Bone research, 2018, 6(1): 9.
        Species: Mouse
      20. Publication: Zhang Z, Zhang H, Chen R, et al. Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses[J]. Molecular Medicine Reports, 2018, 17(5): 7142-7148.
        Species: Mouse
      21. Publication: Deng Z, Li Z, Sun C, et al. The Association Between Inflammation, The Microbiome and Urethane-induced Pulmonary Adenocarcinoma[J]. Oncology Letters, 2018, 15(5): 6352-6360.
        Species: Mouse
      22. Publication: Wang J, Wang L, Zhou J, et al. The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice[J]. Biomedicine & Pharmacotherapy, 2018, 106: 1250-1257.
        Species: Mouse
        Sample Type: SerumandHippocampus tissue
      23. Publication: Ren F, Li J, Wang Y, et al. The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice[J]. Cellular Physiology and Biochemistry, 2018, 47(3): 1084-1094.
        Species: Mouse
        Sample Type: Serum
      24. Publication: SONG Quan Quan, NIU Jing Ping, ZHANG Shu Yu, LIANG Ting Ting, ZHOU Ji, FENG Shan Shan. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice. Biomedical and Environmental Sciences, 2018, 31(10): 757-768.
        Species: Mouse
        Sample Type: Plasma and Heart homogenate
      Show AllShorten
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History

        People Also Bought

        Leave Message

        Message submitted successfully !