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Na+K+-ATPase Activity Assay Kit

Cat.No.:E-BC-K539-M
Detection instrument: Microplate reader(640 nm-680 nm,optimum wavelength: 660 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Na+K+-ATPase decomposes ATP to produce ADP and Phosphorus, and calculates the activity of Na+K+-ATPase by measuring the content of phosphorus.

Performance characteristics

Synonyms	Na+K+-ATPase
Sample type	Serum, plasma, animal tissue
Sensitivity	0.11 μmol Pi/mL/hour
Detection range	0.42-4.99 μmol Pi/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	35 min
Precision	Average inter-assay CV: 2.200%Average intra-assay CV: 2.200%
Other instruments required	Micropipettor, Tubes, Vortex mixer, Incubator, 37℃ Water bath, Centrifuge.
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.42-4.99 μmol Pi/mL/hour).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Rat plasma	1
10% Rat liver tissue homogenate	6-10
10% Rat spleen tissue homogenate	4-8
10% Rat heart tissue homogenate	4-8
10% Mouse brain tissue homogenate	4-8

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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Reviews/Q&A

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Reviews
Q&A

H**********gSubmitted [ Oct 21 2021 ]

Asked: Hi, Is this kit suitable to assess the activity of Na-K ATPase with skeletal muscle homogenates? Any relevant publication?

adminSubmitted [ Oct 21 2021 ]

Answered: The kit can detect animal tissue and skeletal muscle. The citation for this item has not been collected yet. Thank you.

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