Current Position:Home>>Kits>>Metabolism Assays>> NADP+/NADPH Colorimetric Assay Kit (WST-8)
Cat.No.:E-BC-K803-M
Welcome to order from local distributors.
Protocols for metabolism assays
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Synonyms | NADP/NADPH |
Sample type | animal tissu, cells |
Sensitivity | 0.02 μmol/L |
Detection range | 0.02-5.0 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 70 min |
Precision | Average inter-assay CV: 5.500%Average intra-assay CV: 2.100% |
Other instruments required | Test tube, Micropipettor, 37℃ water bath, 10 KD filters tube. |
Other reagents required | Ultrapure water |
Storage | -20℃ |
Valid period | 12 months |
The ratio of NADP+/NADPH was significantly changed in different groups. (P<0.05)
T P Cimmino et al investigate the function of formyl-peptide receptor 2 signaling in anabolic pathways. Ratio of NADP+/NADPH in cell was determined using NADP+/NADPH colorimetric assay kit (E-BC-K803-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.02-5.0 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Jurkat cells |
1 |
Mark cells |
1 |
HCT116 cells |
1 |
293T cells |
1 |
10% Mouse kidney tissue homogenate |
1 |
Hela cells |
1 |
Note: The diluent is reagent 1.
PMID: 36139766 |
Sample: CaLu-6 cell |
DOI: 10.1002/tox.23740 |
|
Sample: Kidney |
DOI: 10.1111/jdi.13690 |
|
Sample: skeletal muscle,heart,pancreas |
DOI: 10.1002/bit.28222 |
|
PMID: 36062959 |