Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K013-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $220
      Can detect 40 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(690-730 nm,optimum wavelength: 715 nm)

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      Detection principle

      Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.

      Performance characteristics

      Synonyms NEFA
      Sample type Animal tissue,cells
      Sensitivity 0.15 mmol/L
      Detection range 0.15-1.5 mmol/L
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 30 min
      Precision Average inter-assay CV: 5.1%Average intra-assay CV: 3.3%
      Other instruments required Vortex mixer, Micropipettor, Multichannel transferpettor (300 μL)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample


      Citations

      1. 2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish
        IF: 3.743
        Journal: BIOMEDICINE & PHARMACOTHERAPY(2020)
        DOI: 10.1016/j.biopha.2020.110138
        PMID: 99999999
        Products: E-BC-K013-M E-BC-K020-M E-EL-0060 
        Sample type: Tissue homogenate
        Reactivity: Fish
      2. Inhibition of adiposity and related metabolic disturbances by polyphenol-rich extract of Boswellia serrata gum through alteration of adipo/cytokine profiles
        IF: 3.304
        Journal: Inflammopharmacology(2018)
        DOI: 10.1007/s10787-018-0519-4
        PMID: 30069718
        Products: E-BC-K013-M 
        Sample type: Serum
        Reactivity: Rat
      3. Inhibition of adiposity and related metabolic disturbances by polyphenol-rich extract of Boswellia serrata gum through alteration of adipo/cytokine profiles
        IF: 3.304
        DOI: 10.1007/s10787-018-0519-4
        PMID: 99999999
        Products: E-BC-K013-M 
        Sample type: Cell lysate
        Reactivity: Human
      4. Anti-obesity potential of Moringa olifera seed extract and lycopene on high fat diet induced obesity in male Sprauge Dawely rats
        IF: 2.802
        Journal: Saudi Journal of Biological Sciences(2020)
        DOI: 10.1016/j.sjbs.2020.06.026
        PMID: 32994733
        Products: E-BC-K013-M 
        Sample type: Serum
        Reactivity: Rat
      5. Combination of Pancreastatin inhibitor PSTi8 with metformin inhibits Fetuin-A in type 2 diabetic mice
        Journal: Heliyon(2020)
        DOI: 10.1016/j.heliyon.2020.e05133
        PMID: 33033766
        Products: E-BC-K013-M 
        Sample type: Plasma,Tissue homogenate
        Reactivity: Mouse
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      MSDS for ELISA ISO 9001

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