Current Position:Home>>Kits>>Metabolism Assays>> Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

Cat.No.:E-BC-K013-M
Detection instrument: Microplate reader(690-730 nm,optimum wavelength: 715 nm)

Detection method: Colorimetric method

To Purchase E-BC-K013-M

Size:

96T
48T

Price: $220

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.

Performance characteristics

Synonyms	NEFA
Sample type	Animal tissue
Sensitivity	0.15 mmol/L
Detection range	0.15-1.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 5.100%Average intra-assay CV: 3.300%
Other instruments required	Vortex mixer, Micropipettor, Multichannel transferpettor (300 μL)
Storage	2-8℃
Valid period	12 months

Images

Intracellular free fatty acid was significantly reduced in shM5-1 and shM5-2 group. (P<0.0001)

H Peng et al investigate the effect m6A in 18S rRNA of promoting fatty acid metabolism and oncogenic transformation. Non-esterified Free Fatty Acid in Human cell was determined using NEFA colorimetric assay kit (E-BC-K013-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.15-1.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Rat liver tissue homogenate	1
Rat heart tissue homogenate	1
Rat kidney tissue homogenate	1
Mouse liver tissue homogenate	1

Note: The diluent is Reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

BIOMATERIALS (2022) IF: 15.304
Self-amplified ROS production from fatty acid oxidation enhanced tumor immunotherapy by atorvastatin/PD-L1 siRNA lipopeptide nanoplexes

DOI: 10.1016/j.biomaterials.2022.121902

Sample: B16-F10 cell,CT-26 cell
SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
Combined effect of polystyrene microplastics and bisphenol A on the human embryonic stem cells-derived liver organoids: The hepatotoxicity and lipid accumulation

DOI: 10.1016/j.scitotenv.2022.158585

PMID: 36089014
Sample: liver organoids (LOs)
SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids

DOI: 10.1016/j.scitotenv.2021.150328

PMID: 34571217
Sample: liver organoids (LOs)
PEDIATRIC ALLERGY AND IMMUNOLOGY (2021) IF: 6.377
The dispersion and utilization of lipid droplets mediates respiratory syncytial virus-induced airway hyperresponsiveness

DOI: 10.1111/pai.13651

Sample: Beas-2b cell
Nutrients (2022) IF: 5.719
The Allium Derivate Propyl Propane Thiosulfinate Exerts Anti-Obesogenic Effects in a Murine Model of Diet-Induced Obesity

DOI: 10.3390/nu14030440

PMID: 35276798
Sample: plasma
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish

DOI: 10.1016/j.biopha.2020.110138

Sample: Tissue homogenate
Clinical Epidemiology (2021) IF: 4.79
Cardiovascular Disease Prediction by Machine Learning Algorithms Based on Cytokines in Kazakhs of China

DOI: 10.2147/CLEP.S313343

PMID: 34135637
Sample: blood
INFLAMMOPHARMACOLOGY (2018) IF: 3.304
gum through alteration of adipo/cytokine profiles

DOI: 10.1007/s10787-018-0519-4

Sample: Serum
Heliyon (2020) IF: 3.776
Combination of Pancreastatin inhibitor PSTi8 with metformin inhibits Fetuin-A in type 2 diabetic mice

DOI: 10.1016/j.heliyon.2020.e05133

PMID: 33033766
Sample: Plasma,Tissue homogenate
Journal of Oleo Science (2022) IF: 1.601
Protective Effect of Nimbolide against High Fat Diet-induced Obesity in Rats via Nrf2/HO-1 Pathway

DOI: 10.5650/jos.ess21389

PMID: 35491096
Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

M*****iSubmitted [ Oct 27 2022 ]

Asked: Is it required to use fresh sample?

adminSubmitted [ Oct 27 2022 ]

Answered: Yes, the samples should be fresh collected and detect within 24 hours.

M*****iSubmitted [ Sep 23 2022 ]

Asked: Can this kit be used to measure the non-esterified free fatty acids (NEFA) content in rat serum sample?

adminSubmitted [ Sep 23 2022 ]

Answered: Unfortunately, this kit is not suitable for serum sample.You can choose E-BC-K013-S to detect rat serum sample.

... Show All Show Less

Cat. No	E-BC-K013-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha