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Oxalate (Oxalic Acid) Colorimetric Assay Kit

Cat.No.:E-BC-K892-M
Detection instrument: Micropipettor, Microplate reader (545-555 nm, optimum wavelength: 550 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. Under the action of POD, hydrogen peroxide reacts with chromogenic substances to produce colored products. There is a specific absorption peak at 550 nm, and the color depth is proportional to the content of oxalate.

Performance characteristics

Sample type	animal urine, serum, plasma， plant tissue
Sensitivity	0.02 mmol/L
Detection range	0.02-1 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	35 min
Precision	Average inter-assay CV: 4.700%Average intra-assay CV: 3%
Other instruments required	Micropipettor, Centrifuge, 37℃ incubator
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.02-1 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human urine	1-2
Human plasma	1
10% Epipremnum aureum tissue homogenate	2-3
Rats plasma	1

Note: The diluent is double distilled water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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