Alternate Names	T-cell surface glycoprotein CD8 alpha chain,CD8A,T-lymphocyte differentiation antigen T8/Leu-2,MAL
Swissprot	P01731
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Zamoyska R. 1994. Immunity 1:243. 3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Product Details

Form	Liquid
Clone No.	53-6.7(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	PE Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832D]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD8a Antibody[53-6.7]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
Biotin Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
FITC Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD8a Antibody[53-6.7]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
PE Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
APC Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
PerCP Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD8a Antibody[53-6.7]	25μg, 100μg	Check More

Citations

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2022) IF: 12.658
Cyclin G2 in macrophages triggers CTL-mediated antitumor immunity and antiangiogenesis via interferon-gamma

DOI: 10.1186/s13046-022-02564-2

Sample: lung cancer (LLC) cells，cytotoxic T lymphocytes (C
ACS Applied Materials & Interfaces (2022) IF: 10.383
Self-Activated Cascade Biocatalysis of Glucose Oxidase–Polycation–Iron Nanoconjugates Augments Cancer Immunotherapy

DOI: 10.1021/acsami.2c04894

Sample: Spleens
Frontiers in Immunology (2022) IF: 7.561
Targeting Glutamine Metabolism Ameliorates Autoimmune Hepatitis via Inhibiting T Cell Activation and Differentiation.

DOI: 10.3389/fimmu.2022.880262

Sample: splenocytes
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53
Epigallocatechin-3-gallate protects immunity and liver drug-metabolism function in mice loaded with restraint stress

DOI: 10.1016/j.biopha.2020.110418

Sample: Whole Blood
Marine Drugs (2022) IF: 5.118
In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

DOI: 10.3390/md20030197

PMID: 35323496
Sample: Dendritic cells (DCs)
INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
Dioscin modulates macrophages polarization and MDSCs differentiation to inhibit tumorigenesis of colitis-associated colorectal cancer

DOI: 10.1016/j.intimp.2023.109839

Sample: blood,spleen,myeloid-derived suppressor cells (MDS
TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY (2020) IF: 1.05
Formaldehyde inhibits development of T lymphocytes in mice

DOI: 10.1080/02772248.2020.1815202

Sample: Cell culture medium

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