Alternate Names	HLA class II histocompatibility antigen DR alpha/ DRB1-15 beta chain,MHC class II antigen DRA,HLA-DRA1/DRB1,DRA/DRB1
Swissprot	P01903,P01911
References	1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177. 2. Terstappen L, et al. 1990. J. Leuk. Biol. 48:138. 3. Edwards J, et al. 1985. J. Immunol. 137:490. 4. van Es A, et al. 1984. Transplantation 37:65. 5. O'Doherty U, et al. 1994. Immunology 82:487. 6. Thomas R, et al. 1994. J. Immunol. 153:4016. 7. Grouard G, et al. 1996. Nature 384:364.

Product Details

Form	Liquid
Clone No.	L243(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a, κ
Isotype Control	PE/Cyanine5.5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802I]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5.5
	PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human HLA-DR Antibody[L243]	50μg, 500μg, 1mg	Check More
Purified Anti-Human HLA-DR Antibody[L243]	25μg, 100μg	Check More
Biotin Anti-Human HLA-DR Antibody[L243]	25μg, 100μg	Check More
FITC Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human HLA-DR Antibody[L243]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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