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PE/Elab Fluor® 594 Anti-Mouse IFN-γ Antibody[XMG1.2]

Uniprot : P01580
  • Cat.No.:E-AB-F1101P

  • Applications: ICFCM
  • Isotype: Rat IgG1, κ
  • Host: Rat

To Purchase E-AB-F1101P

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Antigen Details

Alternate Names Interferon gamma,Ifng,IFN-gamma,IFNγ
Swissprot P01580
Gene ID 15978
References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Product Details

Form Liquid
Clone No. XMG1.2(See Other Available Formats)
Host Rat
Isotype Rat IgG1, κ
Isotype Control PE/Elab Fluor® 594 Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09822P]
Reactivity Mouse
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Elab Fluor® 594
PE/Elab Fluor® 594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Protocols & Troubleshootings

Related Protocols Staining Intracellular Antigens for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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