Alternate Names	Tumor necrosis factor receptor superfamily member 5,Cd40,B-cell surface antigen CD40,Bp50,CD40L receptor,CD40,Tnfrsf5
Swissprot	P25942
References	1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881. 2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.

Product Details

Form	Liquid
Clone No.	3A8(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a, κ
Isotype Control	[Product E-AB-F09802F]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PerCP
	PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Human CD40 Antibody[3A8]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD40 Antibody[3A8]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD40 Antibody[3A8]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD40 Antibody[3A8]	20Tests, 100Tests, 100Tests×2	Check More

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Frontiers in Immunology (2021) IF: 7.561

The Impact of Rubella Virus Infection on a Secondary Inflammatory Response in Polarized Human Macrophages

DOI: 10.3389/fimmu.2021.772595
PMID: 34975859	Sample: Monocyte

Biomedicines (2022) IF: 6.081

CD14 Is Involved in the Interferon Response of Human Macrophages to Rubella Virus Infection

DOI: 10.3390/biomedicines10020266
	Sample: Macrophages(MΦ)

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