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PerCP Anti-Human CD40 Antibody[3A8]

  • Cat.No.:E-AB-F1037F

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse

To Purchase E-AB-F1037F

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $75
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Antigen Details

Alternate Names Tumor necrosis factor receptor superfamily member 5,Cd40,B-cell surface antigen CD40,Bp50,CD40L receptor,CD40,Tnfrsf5
Swissprot P25942
References
1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.

Product Details

Form Liquid
Clone No. 3A8(See Other Available Formats)
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control [Product E-AB-F09802F]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PerCP
PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human CD40 Antibody[3A8] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD40 Antibody[3A8] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD40 Antibody[3A8] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human CD40 Antibody[3A8] 20Tests, 100Tests, 100Tests×2 Check More

Citations

  1. Biomedicines (2022) IF: 6.081
    CD14 Is Involved in the Interferon Response of Human Macrophages to Rubella Virus Infection

    DOI: 10.3390/biomedicines10020266

    Sample: Macrophages(Mφ)
  2. Frontiers in Immunology (2021) IF: 7.561
    The Impact of Rubella Virus Infection on a Secondary Inflammatory Response in Polarized Human Macrophages

    DOI: 10.3389/fimmu.2021.772595

    PMID: 34975859

    Sample: Monocyte

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