Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PerCP/Cyanine5.5 Anti-Human CD197/CCR7 Antibody[G043H7]
Cat.No.:E-AB-F1159J
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Alternate Names | CMKBR7, EBI1, EVI1,CCR-7,CDw197 |
Swissprot | P32248 |
References |
1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9. 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8. |
Form | Liquid |
Clone No. | G043H7(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG2a, κ |
Isotype Control | PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802J] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PerCP/Cyanine5.5 |
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5.5 Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 647 Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® Violet 450 Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® Red 780 Anti-Human CD197/CCR7 Antibody[G043H7] | 20Tests, 100Tests, 100Tests×2 | Check More |