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Peroxidase (POD) Activity Assay Kit (Plant samples)

Cat:E-BC-K227-M Citations (3)
Manual MSDS

Price: $ 240

Price: $ 160

Size:
96T 48T
Quantity:
  • Sample Type: plant tissue
  • Detection Instrument: Microplate reader(410 nm-430 nm,optimum wavelength: 420 nm)
  • Reasearch Areas: Oxidative stress
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For research use only. Order now, ship in 3 days

Product Details
Detection Principle Plant peroxidase, a member of the superfamily of peroxidase, catalyzes the redox reaction between H2O2 and various reductants. The plant peroxidase has the same general structure and consists of iron porphyrin IX and ten α-helixes. Based on the difference of primary structure, the superfamily of plant peroxidase can be divided into three types: class I (intracellular type), class II (extracellular type of fungi) and class III (secreted type of plant).
Synonyms POD
Sample Type plant tissue
Sensitivity 0.01 U/mL
Detection Range 0.01–100 U/mL
Detection Method Colorimetric method
Assay Time 60 min
Precision inter-assay CV: 5% | intra-assay CV: 3.2%
Other Reagents Required PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
10% Green pepper tissue homogenate 1
10% Chive leaf tissue homogenate 1
10% Photinia leaf tissue homogenate 1
10% Epipremnum aureum tissue homogenate 1
10% Mushrooms tissue homogenate 1
10% White radish tissue homogenate 1
The diluent is PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.