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Pg(Progesterone) ELISA Kit

Citations(67)

  • Cat.No.:E-EL-0154

  • Reactivity: Universal
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Manual MSDS

To Purchase E-EL-0154

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.31-20 ng/mL
Sensitivity 0.15 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Pg. During the reaction, Pg in the sample or standard competes with a fixed amount of Pg on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Pg. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pg in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Pg were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Pg were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.56 2.22 8.78 0.54 2.01 8.55
Standard deviation 0.05 0.14 0.53 0.05 0.14 0.53
CV (%) 8.39 6.42 5.98 9.35 7.04 6.21

Recovery

The recovery of Pg spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 80-94 84
EDTA plasma(n=8) 82-96 87
Urine(n=8) 85-109 95

Linearity

Samples were spiked with high concentrations of Pg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaSignal Transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
elisa assay procedure 2

2. Aspirate and wash the plate for 3 times
elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 5

5. Add 50μL Stop Solution
elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Journal of Extracellular Vesicles (2020) IF: 14.976
    Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy

    DOI: 10.1080/20013078.2020.1795364
    Sample: BeWo cell
  2. Redox Biology (2021) IF: 11.799
    Melatonin protects against environmental stress-induced fetal growth restriction via suppressing ROS-mediated GCN2/ATF4/BNIP3-dependent mitophagy in placental trophoblasts

    DOI: 10.1016/j.redox.2021.101854

    PMID: 33454563

    		 Sample: placentae tissue
  3. JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588
    Di (2-ethyl-hexyl) phthalate disrupts placental growth in a dual blocking mode

    DOI: 10.1016/j.jhazmat.2021.126815
    Sample: serum
  4. CARBOHYDRATE POLYMERS (2022) IF: 9.381
    Butylated starch alleviates polycystic ovary syndrome by stimulating the secretion of peptide tyrosine-tyrosine and regulating faecal microbiota

    DOI: 10.1016/j.carbpol.2022.119304

    PMID: 35422283

    		 Sample: serum
  5. ENVIRONMENTAL POLLUTION (2023) IF: 9.988
    Paternal genetic intergenerational and transgenerational effects of cadmium exposure on hormone synthesis disorders in progeny ovarian granulosa cells

    DOI: 10.1016/j.envpol.2023.121175
    Sample: Serum
  6. ENVIRONMENTAL POLLUTION (2022) IF: 9.988
    Betulinic acid alleviates zearalenone-induced uterine injury in mice

    DOI: 10.1016/j.envpol.2022.120435

    PMID: 36257561

    		 Sample: serum
  7. International Journal of Nanomedicine (2022) IF: 7.033
    Biocompatible Assessment of Erythrocyte Membrane-Camouflaged Polymeric PLGA Nanoparticles in Pregnant Mice: Both on Maternal and Fetal/Juvenile Mice

    DOI: 10.2147/IJN.S384906
    Sample: serum
  8. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129
    Maternal exposure to cadmium from puberty through lactation induces abnormal reproductive development in female offspring

    DOI: 10.1016/j.ecoenv.2022.113927

    PMID: 35908533

    		 Sample: serum
  9. ENVIRONMENT INTERNATIONAL (2020) IF: 7.577
    Environmental cadmium exposure induces fetal growth restriction via triggering PERK-regulated mitophagy in placental trophoblasts

    DOI: 10.1016/j.envint.2020.106319

    PMID: 33348103

    		 Sample: serum, amniotic fluid,cell medium,Cell lysate
  10. ENVIRONMENT INTERNATIONAL (2020) IF: 7.577
    Bisphenol A promotes autophagy in ovarian granulosa cells by inducing AMPK/mTOR/ULK1 signalling pathway

    DOI: 10.1016/j.envint.2020.106298

    PMID: 33387880

    		 Sample: Cell culture supernatant,Serum
  11. Antioxidants (2022) IF: 7.675
    Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

    DOI: 10.3390/antiox11101879

    PMID: 36290602

    		 Sample: serum
  12. International Journal of Nanomedicine (2021) IF: 6.4
    Development and Evaluation of Curcumin Encapsulated Self-assembled Nanoparticles as Potential Remedial Treatment for PCOS in a Female Rat Model

    DOI: 10.2147/IJN.S302161

    PMID: 34531655

    		 Sample: serum
  13. CHEMICO-BIOLOGICAL INTERACTIONS (2023) IF: 5.168
    Protective effect of eicosapentaenoic acid against estradiol valerate-induced endometrial hyperplasia via modulation of NF-κB/HIF-1α/VEGF signaling pathway in rats

    DOI: 10.1016/j.cbi.2023.110399
    Sample: serum
  14. Reproductive Biology and Endocrinology (2021) IF: 5.211
    Irisin deletion induces a decrease in growth and fertility in mice

    DOI: 10.1186/s12958-021-00702-7

    PMID: 33581723

    		 Sample: Serum
  15. Food & Function (2022) IF: 5.396
    Lactiplantibacillus plantarum CCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism

    DOI: 10.1039/D1FO01744F

    PMID: 35044398

    		 Sample: serum
  16. ENVIRONMENTAL POLLUTION (2019) IF: 5.714
    Exposure to DEHP or its metabolite MEHP promotes progesterone secretion and inhibits proliferation in mouse placenta or JEG-3 cells

    DOI: 10.1016/j.envpol.2019.113593
    Sample: Cell culture medium
  17. Cell Death & Disease (2017) IF: 5.965
    ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats

    DOI: 10.1038/cddis.2017.494
    Sample: Serum
  18. Frontiers in Pharmacology (2022) IF: 5.988
    Andrographolide exerted anti-inflammatory effects thereby reducing sex hormone synthesis in LPS-induced female rats, but had no effect on hormone production in healthy ones.

    DOI: 10.3389/fphar.2022.980064
    Sample: serum
  19. POULTRY SCIENCE (2022) IF: 4.014
    Corticosterone stage-dependently inhibits progesterone production presumably via impeding the cAMP-StAR cascade in granulosa cells of chicken preovulatory follicles

    DOI: 10.1016/j.psj.2022.102379
    Sample: granulosa cells (GCs)
  20. JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020) IF: 4.018
    Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice

    DOI: 10.1016/j.jri.2020.103240

    PMID: 33166807

    		 Sample: Serum
  21. JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2022) IF: 4.054
    Human umbilical cord mesenchymal stem cell-derived exosomes inhibit ovarian granulosa cells inflammatory response through inhibition of NF-κB signaling in polycystic ovary syndrome

    DOI: 10.1016/j.jri.2022.103638

    PMID: 35588629

    		 Sample: granulosa cell.
  22. BIOLOGY OF REPRODUCTION (2022) IF: 4.161
    Role of progesterone on dexamethasone-induced alterations in placental vascularization and progesterone receptors in rats

    DOI: 10.1093/biolre/ioac192
    Sample: serum
  23. Frontiers in Pharmacology (2020) IF: 4.225
    Chemical Fingerprint Analysis and Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics Study of the Protective Effect of Buxue Yimu Granule in Medical-Induced Incomplete Abortion Rats

    DOI: 10.3389/fphar.2020.578217

    PMID: 33328985

    		 Sample: serum
  24. JOURNAL OF BIOLOGICAL CHEMISTRY (2016) IF: 4.258
    Activation of PPARγ and CD36 expression--the dual pathophysiological roles of progesterone

    DOI: 10.1074/jbc.M116.726737
    Sample: Serum
  25. BIOLOGY OF REPRODUCTION (2022) IF: 4.285
    Pre-implantation triclosan exposure alters uterine receptivity through affecting tight junction protein.

    DOI: 10.1093/biolre/ioac092
    Sample: serum
  26. BIOLOGY OF REPRODUCTION (2021) IF: 4.285
    Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice

    DOI: 10.1093/biolre/ioab040
    Sample: serum
  27. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Network pharmacology-based study on immunomodulatory mechanism of danggui-yimucao herb pair for the treatment of RU486-induced abortion

    DOI: 10.1016/j.jep.2021.114609

    PMID: 34508802

    		 Sample: serum
  28. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Evaluation of possible effects of Persea americana seeds on female reproductive hormonal and toxicity profile

    DOI: 10.1016/j.jep.2021.113870

    PMID: 33484907

    		 Sample: Serum
  29. Human Cell (2022) IF: 4.374
    Repeated controlled ovarian stimulation-induced ovarian and uterine damage in mice through the PI3K/AKT signaling pathway

    DOI: 10.1007/s13577-022-00829-8
    Sample: serum
  30. TOXICOLOGY LETTERS (2021) IF: 4.374
    Copper exposure disrupts ovarian steroidogenesis in human ovarian granulosa cells via the FSHR/CYP19A1 pathway and alters methylation patterns on the SF-1 gene promoter

    DOI: 10.1016/j.toxlet.2021.12.002

    PMID: 34871762

    		 Sample: ovarian granulosa cell
  31. MOLECULES (2021) IF: 4.412
    The Physicochemical Characteristics of Gelam Honey and Its Outcome on the Female Reproductive Tissue of Sprague–Dawley Rats: A Preliminary Study

    DOI: 10.3390/molecules26113346
    Sample: serum
  32. TOXICOLOGY (2022) IF: 4.571
    A metabolomic approach to study triptolide-induced ovarian damage in rats

    DOI: 10.1016/j.tox.2022.153351

    PMID: 36272568

    		 Sample: ovarian granulosa cells
  33. Biochimica et Biophysica Acta-Gene Regulatory Mechanisms (2019) IF: 4.599
    CircINHA resists granulosa cell apoptosis by upregulating CTGF as a ceRNA of miR-10a-5p in pig ovarian follicles

    DOI: 10.1016/j.bbagrm.2019.194420

    PMID: 31476383

    		 Sample: Cell sample
  34. Metallomics (2022) IF: 4.636
    Transcriptomic analysis reveals the effects of maternal selenium deficiency on placental transport, hormone synthesis, and immune response in mice

    DOI: 10.1093/mtomcs/mfac062

    PMID: 36002020

    		 Sample: serum,placenta
  35. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872
    Cadmium exposure during prenatal development causes progesterone disruptors in multiple generations via steroidogenic enzymes in rat ovarian granulosa cells

    DOI: 10.1016/j.ecoenv.2020.110765
    Sample: Cell culture supernatant
  36. REPRODUCTIVE TOXICOLOGY (2022) IF: 3.143
    Bisphenol A exposure advances puberty onset by changing Kiss1 expression firstly in arcuate nucleus at juvenile period in female rats

    DOI: 10.1016/j.reprotox.2022.04.005

    PMID: 35429613

    		 Sample: serum
  37. NEUROPEPTIDES (2022) IF: 3.152
    Effects of intracerebroventricular and intravenous administration of Kisspeptin-54 and Gonadotropin-releasing hormone agonist in rats with ovarian hyperstimulation

    DOI: 10.1016/j.npep.2022.102298

    PMID: 36367482

    		 Sample: serum
  38. Reproductive Biology and Endocrinology (2020) IF: 3.235
    Genipin improves reproductive health problems caused by circadian disruption in male mice

    DOI: 10.1186/s12958-020-00679-9

    PMID: 33308222

    		 Sample: Cell culture supernatant
  39. TOXICOLOGY AND APPLIED PHARMACOLOGY (2020) IF: 3.347
    2,5-Hexanedione influences primordial follicular development in cultured neonatal mouse ovaries by interfering with the PI3K signaling pathway via miR-214-3p

    DOI: 10.1016/j.taap.2020.115335

    PMID: 33197454

    		 Sample: Cell culture supernatant
  40. BEHAVIOURAL BRAIN RESEARCH (2022) IF: 3.352
    Postpartum depression in rats causes poor maternal care and neurochemical alterations on dams and long-lasting impairment in sociability on the offspring

    DOI: 10.1016/j.bbr.2022.114082

    PMID: 36041571

    		 Sample: serum
  41. POULTRY SCIENCE (2022) IF: 3.352
    Effects of stocking density on ovarian development and maturation during the rearing period in Shan-ma ducks

    DOI: 10.1016/j.psj.2022.101809

    PMID: 35358924

    		 Sample: serum
  42. TOXICOLOGY IN VITRO (2021) IF: 3.5
    Long-term in vitro exposure of human granulosa cells to the mixture of endocrine disrupting chemicals found in human follicular fluid disrupts steroidogenesis

    DOI: 10.1016/j.tiv.2021.105302

    PMID: 34929288

    		 Sample: HGrC1 cell
  43. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020) IF: 3.606
    Protective roles and mechanisms of rosmarinic acid in cyclophosphamide‐induced premature ovarian failure

    DOI: 10.1002/jbt.22591
    Sample: Serum
  44. FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775
    Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats

    DOI: 10.1016/j.fct.2019.110897

    PMID: 31654709

    		 Sample: Serum
  45. REPRODUCTIVE BIOMEDICINE ONLINE (2021) IF: 3.828
    Progesterone partially recovers placental glucose transporters in dexamethasone induced intrauterine growth restriction

    DOI: 10.1016/j.rbmo.2021.10.016
    Sample: serum
  46. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY (2022) IF: 3.963
    Possible changes in trade-off strategy in female lizards (Eremias argus) during hibernation following exposure to chlorantraniliprole: Impact on the HPG axis and the energy mobilization

    DOI: 10.1016/j.pestbp.2022.105059

    PMID: 35715026

    		 Sample: plasma
  47. Evidence-based Complementary and Alternative Medicine (2018) IF: 2.064
    Research on Establishment of Abnormal Phlegmatic Syndrome with Premature Ovarian Failure Rat Model and Effects of Balgham Munziq Treatment

    DOI: 10.1155/2018/3858209
    Sample: serum
  48. MOLECULAR BIOLOGY REPORTS (2022) IF: 2.316
    The effect of progesterone administration on the expression of metastasis tumor antigens (MTA1 and MTA3) in placentas of normal and dexamethasone-treated rats

    DOI: 10.1007/s11033-021-07005-5

    PMID: 35037193

    		 Sample: serum
  49. GENE (2018) IF: 2.498
    Obesity-induced overexpression of miRNA-24 regulates cholesterol uptake and lipid metabolism by targeting SR-B1

    DOI: 10.1016/j.gene.2018.05.072
    Sample: Serum
  50. Veterinary Sciences (2023) IF: 2.518
    Effect of Feeding Pomegranate (Punica granatum) Peel and Garlic (Allium sativum) on Antioxidant Status and Reproductive Efficiency of Female Rabbits

    DOI: 10.3390/vetsci10030179
    Sample: serum
  51. WATER AIR AND SOIL POLLUTION (2021) IF: 2.52
    Effects of Environmental-Related Concentration of Tamoxifen on Hormonal Balance and Reproduction of the Golden Apple Snail, Pomacea canaliculata (Caenogastropoda: Ampullariidae)

    DOI: 10.1007/s11270-021-05472-x
    Sample: sexual organ
  52. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model

    DOI: 10.1155/2021/9968463

    PMID: 34987599

    		 Sample: serum
  53. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion

    DOI: 10.1155/2022/8251503

    PMID: 36212974

    		 Sample: serum
  54. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2022) IF: 2.723
    Cytosine-phosphate-guanine oligodeoxynucleotides regulate the cell cycle, apoptosis, and steroidogenesis of mouse ovarian granulosa cells by targeting inhibin alpha (1 ~ 32) fragments

    DOI: 10.1007/s11626-022-00662-1

    PMID: 35378691

    		 Sample: mGCs
  55. THERIOGENOLOGY (2021) IF: 2.74
    The pharmacokinetics of letrozole and its effect on gonadotropins in anestrous ewes

    DOI: 10.1016/j.theriogenology.2021.09.033

    PMID: 34628085

    		 Sample: plasma
  56. PLoS One (2017) IF: 2.806
    Insulin resistance enhances the mitogen-activated protein kinase signaling pathway in ovarian granulosa cells

    DOI: 10.1371/journal.pone.0188029
    Sample: Cell culture supernatant
  57. Food Science & Nutrition (2021) IF: 2.863
    Metabolomics study of Angelica sinensis (Oliv.) Diels on the abnormal uterine bleeding rats by ultra-performance liquid chromatography–quadrupole–time-of-flight mass spectrometry analysis

    DOI: 10.1002/fsn3.2605
    Sample: serum
  58. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019) IF: 2.928
    Expression of FKBP52 in the ovaries of PCOS rats

    DOI: 10.3892/ijmm.2018.3998

    PMID: 30483787

    		 Sample: Serum
  59. REPRODUCTION IN DOMESTIC ANIMALS (2018) IF: 1.422
    Methylation pattern variation between goats and rats during the onset of puberty

    DOI: 10.1111/rda.13172
    Sample: Serum
  60. Toxicology Research (2019) IF: 1.593
    Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells

    DOI: 10.1039/C8TX00263K

    PMID: 31160972

    		 Sample: Cell culture medium
  61. ANIMAL REPRODUCTION SCIENCE (2020) IF: 1.66
    Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance

    DOI: 10.1016/j.anireprosci.2020.106531

    PMID: 32828406

    		 Sample: Serum
  62. REPRODUCTION FERTILITY AND DEVELOPMENT (2019) IF: 1.723
    The distribution and expression of vascular endothelial growth factor A (VEGFA) during follicular development and atresia in the pig

    DOI: 10.1071/RD18508
    Sample: follicular fluid
  63. REPRODUCTION IN DOMESTIC ANIMALS (2022) IF: 1.858
    Induction of ovulation using repeated doses of sulpiride, a dopamine antagonist, in ewe-lambs

    DOI: 10.1111/rda.14295
    Sample: plasma
  64. Journal of Zhejiang University-SCIENCE B (2019) IF: 1.879
    Improvement in post-partum uterine involution in rats treated with Apios americana

    DOI: 10.1631/jzus.B1800475
    Sample: serum
  65. Veterinary World (2020)
    Variability of serum reproductive hormones in cows presenting various reproductive conditions in semi-arid areas of the North West Province, South Africa

    DOI: 10.14202/vetworld.2020.502-507

    PMID: 32367956

    		 Sample: Serum
  66. Veterinary World (2022)
    Potential use of Indonesian basil (Ocimum basilicum) maceration to increase estradiol and progesterone synthesis and secretion to improve prenatal growth of offspring using female albino rats as an animal model

    DOI: 10.14202/vetworld.2022.1197-1207
    Sample: serum
  67. Journal of Ayurveda and Integrative Medicine (2020)
    Hormone-balancing and protective effect of combined extract of Sauropus androgynus and Elephantopus scaber against Escherichia coli-induced renal and hepatic necrosis in pregnant mice

    DOI: 10.1016/j.jaim.2020.09.001
    Sample: Serum
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Reviews/Q&A

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  • Reviews
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Verified Customer

Z*ySubmitted [ Jun 01 2019 ]

  • Species:Human
  • Sample:serum
  • Sample source:serum
  • Detection result:good
  • Description:This ELISA Kit was used to assay normal human(women and men) serum by us. We operated procedure as Elabscience,s the instruction manual provided and the experimental results were very good------when the human serums were detected without dilution, the testing results were in the range of standard curve, what’s more, It is very near compared with the clinical assay kit! The Pg level of normal men serum is 0.1-3.5 ng/mL and the follicular phase women serum is 0.1-2.1 ng/mL.
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Q

A*kSubmitted [ Feb 04 2023 ]

Asked: Is it use for bovine? This kit applicable or not?

Reply

A

adminSubmitted [ Feb 04 2023 ]

Answered: This kit can be used for bovine samples, we have validated the kit with normal bovine serum/plasma samples, the reference values were about 0.49-3.97ng/mL.

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