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Phenylalanine Ammonia Lyase (PAL) Activity Assay Kit

Cat.No.:E-BC-K522-S
Detection instrument: Spectrophotometer(290 nm)

Detection method: Colorimetric method

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Detection principle

Phenylalanine ammonia lyase (PAL) can catalyze L-phenylalanine to produce trans-cinnamic acid and ammonia, and trans-cinnamic acid has the maximum absorption peak at 290 nm. PAL activity can be calculated by measuring the increase of OD value at 290 nm.

Performance characteristics

Synonyms	PAL
Sample type	Serum, plasma, animal tissue, cells
Sensitivity	0.78 U/g tissue
Detection range	0.78-156 U/g tissue
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 4.600%Average intra-assay CV: 3.100%
Other instruments required	Tubes, Micropipettor, Vortex mixer, 37°C Incubator
Storage	2-8℃
Valid period	12 months

Images

No significant change of PAL activity can be observed after serving with MgONPs.

ZL Wang et al investigate the toxicity effects and mechanisms of MgO nanoparticles on the oomycete pathogen. Phenylalnine ammonia lyase (PAL) activity of potato tissue was determined using PAL activity assay kit (E-BC-K522-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.78-156 U/g tissue).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Epipremnum aureum tissue homogenate	1
10% Carrot tissue homogenate	1
10% Green pepper tissue homogenate	1
10% Corn grain tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Toxics (2022) IF: 4.472
Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and its Host Solanum tuberosum

DOI: 10.3390/toxics10100553

PMID: 36287834
Sample: leaf

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