Phospho-AKT1 (Ser473) Monoclonal Antibody

Uniprot : P31749
    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
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    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
    • methyl antibody-Elabscience
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      Catalog number:E-AB-51038

      Synonyms:AKT 1,AKT,AKT1,AKT1,MGC99656,PKB,PKB-ALPHA,PRKBA,Protein Kinase B Alpha,Protein kinase B,Proto-oncogene c-Akt,RAC Alpha,RAC,RAC-alpha serine/threonine-protein kinase,RAC-PK-alpha

      Size:
      • 20μL
      • 60μL
      • 120μL
      • 200μL
      Qty:
      - +
      Price: $69

      Host: Mouse

      Reactivity: H

      Applications: WB,IHC-p

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Overview

      Synonyms AKT 1,AKT,AKT1,AKT1,MGC99656,PKB,PKB-ALPHA,PRKBA,Protein Kinase B Alpha,Protein kinase B,Proto-oncogene c-Akt,RAC Alpha,RAC,RAC-alpha serine/threonine-protein kinase,RAC-PK-alpha
      Swissprot P31749
      Source Mouse
      Reactivity Human
      Immunogen Synthetic Peptide of Phospho-Akt (S473)
      Application WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R217)
      Recommended dilution WB 1:1000-2000, IHC 1:100-200
      Concentration 1 mg/mL
      Clonality Monoclonal

      Properties

      Cellular localization Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
      Tissue specificity Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
      Clone No. Clone:7F9
      Isotype IgG
      Purification Protein A purification
      Storage instructions Store at -20℃. Avoid freeze / thaw cycles.
      Storage buffer PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
      Background The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

      Images

      methyl antibody-Elabscience

      Western Blot analysis of PC3 cells using Phospho-AKT1 (Ser473) Monoclonal Antibody at dilution of 1:1000.

      methyl antibody-Elabscience

      Immunohistochemistry of paraffin-embedded Human lung carcinoma tissue using Phospho-AKT1 (Ser473) Monoclonal Antibody at dilution of 1:200.

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