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Phospho-EEF2K (Ser366) Polyclonal Antibody

Cat:E-AB-21142
Manual MSDS

Price: $ 399

Price: $ 240

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Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Rat
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:Hela
Dilution

WB 1:500-1:2000, IHC 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366
Abbre eEF2K (phospho Ser366)
Synonyms C86191;Calcium/calmodulin dependent eukaryotic elongation factor 2;Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase;Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase;Calmodulin dependent protein kinase III;cb365;EC 2.7.11.20;eEF 2 kinase;eEF 2K;eEF-2 kinase;eEF-2K;Eef2k;EEF2K protein;EF2K;Elongation factor 2 kinase;Eukaryotic elongation factor 2 kinase;fa04b08;HSU93850;kinase eEF2K;MGC45041;SMEF2K;wu:fa04b08;zgc:100973
Swissprot
Calculated MW 82kDa
Observed MW 105kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytosol,Other locations:cytoplasm,dendritic spine,postsynaptic density
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Epigenetics and Nuclear Signaling; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.