Phosphofructokinase(also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.

Performance characteristics

Synonyms	PFK
Sample type	serum, plasma, tissue and cell sample
Sensitivity	0.27 U/L
Detection range	0.27-32.29 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	15 min
Precision	Average inter-assay CV: 8%Average intra-assay CV: 3%
Other instruments required	Centrifuge
Other reagents required	Normal saline (0.9% NaCl)
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K234-M	Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)
E-BC-K011-M	Glucose-6-phosphate (G6P) Colorimetric Assay Kit
E-BC-K056-M	Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

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Cat. No	E-BC-K612-M
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