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Phosphofructokinase(PFK)Activity Assay Kit

  • Cat.No.:E-BC-K612-M

  • Detection instrument: Microplate reader (330-350 nm, optimum wavelength: 340 nm)

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Detection principle

Phosphofructokinase(also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.

Performance characteristics

Synonyms PFK
Sample type serum, plasma, tissue and cell sample
Sensitivity 0.27 U/L
Detection range 0.27-32.29 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 15 min
Precision Average inter-assay CV: 8%Average intra-assay CV: 3%
Other instruments required Centrifuge
Other reagents required Normal saline (0.9% NaCl)
Storage -20℃
Valid period 12 months



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