Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit (E-BC-K056-M)
For research use only.
| Detection Principle | Under the presence of G6PDH, glucose-6-phosphoric acid is oxidized to 6-PG. In this reaction, NADP+ is reduced to NADPH. Under the action of electron coupling reagent 1-MPMS, NADPH reducts wST-8 to form orange formazan, which has the maximum absorption peak at about 450 nm. Formazan generated in the reaction system is proportional to the activity of G6DPH in the sample. |
| Synonyms | G-6-PD;G6PDH |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Disulfidptosis |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 U/L |
| Detection Range | 0.01-50 U/L |
| Precision | inter-assay CV: 5.8% | intra-assay CV: 2.1% |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Rabbit serum | 1 |
| Cynomolgus monkey | 1 |
| 10% Mouse liver tissue homogenate | 3-5 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 5-10 |
| 10% Mouse brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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