Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit (E-BC-K056-M)
For research use only.
| Detection Principle | Under the presence of G6PDH, glucose-6-phosphoric acid is oxidized to 6-PG. In this reaction, NADP+ is reduced to NADPH. Under the action of electron coupling reagent 1-MPMS, NADPH reducts wST-8 to form orange formazan, which has the maximum absorption peak at about 450 nm. Formazan generated in the reaction system is proportional to the activity of G6DPH in the sample. |
| Synonyms | G-6-PD;G6PDH |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Disulfidptosis |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 U/L |
| Detection Range | 0.01-50 U/L |
| Precision | inter-assay CV: 5.8% | intra-assay CV: 2.1% |
| Sample Volume | 100 μL |
| Assay Time | 20 min |
Note: Data for this module come from published literature that cites this product, displaying other products used together in the same research paper.
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Rabbit serum | 1 |
| Cynomolgus monkey | 1 |
| 10% Mouse liver tissue homogenate | 3-5 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 5-10 |
| 10% Mouse brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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