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Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method)

Cat.No.:E-BC-K245-S
Detection instrument: Spectrophotometer(660 nm)

Detection method: Colorimetric method

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Detection principle

Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.

Performance characteristics

Synonyms	Pi
Sample type	Serum,plasma,tissue
Sensitivity	0.005 mmol/L
Detection range	0.005-2.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 1.300%Average intra-assay CV: 1%
Other instruments required	Water bath, Centrifuge, Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Images

No significant change was observed in wheat tissue.

A Dede et al investigate the assistance on wheat iron biofortification using endophytic Bacillus altitudinis WR 10. Phosphorus (Pi) level in wheat tissue was determined using phosphorus colorimetric assay kit (E-BC-K245-S).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

International Journal of Nanomedicine (2022) IF: 6.4
A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

DOI: 10.2147/IJN.S351814

PMID: 35299865
Sample: BPN-DFO hydrogel
Frontiers in Nutrition (2021) IF: 6.576
Microbial-Assisted Wheat Iron Biofortification Using Endophytic Bacillus altitudinis WR10.

DOI: 10.3389/fnut.2021.704030

Sample: wheat tissue
Frontiers in Physiology (2023) IF: 4.755
Relative comparison of chronic kidney disease-mineral and bone disorder rat models

DOI: 10.3389/fphys.2023.1083725

Sample: Serum
International Journal of Reproductive Biomedicine (2020)
Association of BsmI variant of vitamin D receptor gene with polycystic ovary syndrome: A case-control study

DOI: 10.18502/ijrm.v13i10.7772

Sample: serum

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