Phosphorus (Pi) Colorimetric Assay Kit (Phospho molybdate method)

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    <
    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K245-S

      Size:
      • 50 Assays
      • 100 Assays
      Qty:
      - +
      Price: $120

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      General information

      Detection significance

      Phosphorus is an important mineral that maintains cellular energy, mineralizes bones, and protects non-bone tissue from calcification. Inorganic phosphorus is the component of DNA, RNA, ATP and phospholipid. Phosphorus is existed in the form of ester and phosphate anion in the whole blood. The concentration of phosphorus is strictly regulated by specific ion transport proteins and hormones.

      Detection principle

      Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.

      Operation procedures

      Operation steps

      1.   The preparation of sample supernatant

            Take 0.1 mL serum (plasma) or 10% tissue homogenate sample, then add 0.4 mL reagent 4, mix fully. Centrifuge at 1100 × g for 10 min and take the supernatant for detection.

       

      2.   The measurement of samples

      1)   Blank tube: Take 0.2 mL of double distilled water to an EP tube.

      Standard tube: Take 0.2 mL of 0.5 mmol/L standard solution to an EP tube.

      Sample tube: Take 0.2 mL of sample supernatant to the EP tubes.

      2)   Add 2.0 mL of Chromogenic agent into each tube of Step 1 and mix fully.

      3)   Incubate the tubes at 37℃ for 30 min, then cooling to room temperature.

      4)   Set the spectrophotometer to zero with double distilled water, and measure the OD at 660 nm with 1 cm optical path quartz cuvette.

      Operation table


       

      Blank tube

      Standard tube

      Sample tube

      Double distilled water (mL)

      0.2

       

       

      0.5 mmol/L standard solution (mL)

       

      0.2

       

      Sample supernatant (mL)

       

       

      0.2

      Chromogenic agent (mL)

      2.0

      2.0

      2.0

      Mix fully, then incubate the tubes at 37 for 30 min, then cooling to room temperature. Set the spectrophotometer to zero with double distilled water, and measure the OD at 660 nm with 1 cm optical path quartz cuvette.

      Performance characteristics

      Technical parameter

      Detection range 0.005-2.0 mmol/L Average inter-assay CV 1.3%
      Sensitivity 0.005 mmol/L Average intra-assay CV 1%
      Average recovery rate 102%

      Standard curve

      Dilute 10 mmol/L standard stock solution with double distilled water to a serial concentration. The recommended dilution gradient is as follows: 2, 1, 0.5, 0.2, 0.1, 0.05, 0 mmol/L. Then carry the assay according to the operation table.

      Plot the standard curve by using OD value of standard and correspondent concentration as y-axis and x-axis respectively. Create the standard curve with graph software (or EXCEL). The concentration of the sample can be calculated according to the formula based on the OD value of sample. The standard curve is: y= ax + b.

      Example analysis

      Detect human serum, human urine (dilute for 2 times), culture supernatant of HepG2 cells and 10% mouse liver tissue homogenate (the concentration of protein is 11.99 gprot/L) according to the protocol, the result is as follows:
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History


        People Also Bought

        Leave Message

        *
        *
        *
        *
        *
        *
        *
        VerifyCode
        Message submitted successfully !