Polyphenol oxidase (PPO) can catalyze phenolic compounds into quinone substances. The latter has specific absorption at 410 nm. The activity of PPO can be calculated indirectly by measuring the OD value at 410 nm.

Performance characteristics

Synonyms	PPO
Sample type	Plant tissue
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 9.800%Average intra-assay CV: 4.600%
Other instruments required	Tubes, Micropipette, Vortex mixer, 100℃ Water bath
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Frontiers in Plant Science (2022) IF: 5.754
MC03g0810, an Important Candidate Gene Controlling Black Seed Coat Color in Bitter Gourd (Momordica spp.).

DOI: 10.3389/fpls.2022.875631

Sample: seed coat

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Cat. No	E-BC-K259-S
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