Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Purified Anti-Mouse CD122/IL-2RB Antibody[5H4]
Cat.No.:E-AB-F1029A
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Alternate Names | Interleukin-2 receptor subunit beta,Il2rb,IL-2 receptor subunit beta, IL-2R subunit beta,IL-2RB,High affinity IL-2 receptor subunit beta,p70-75,CD122 |
Swissprot | P16297 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245. 3. Suzuki H, et al. 1995. Science 268:1472. 4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109. |
Form | Liquid |
Clone No. | 5H4(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833A] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Unconjugated |
How to select the appropriate detection channel through the spectrogram?
CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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