Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	36.24 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human Pg in samples.No significant cross-reactivity or interference between Human Pg and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Performance

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Pg are added to the micro ELISA plate wells. Human Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Progesterone (P4) plays a central role in reproductive events associated with the establishment and maintenance of pregnancy in all mammals. In women, P4 is synthesized by the corpus luteum during the luteal phase of the menstrual cycle and during the first weeks of pregnancy, and then mainly by the placenta until term. P4 is needed during early embryonic development for implantation and placentation; insufficient P4 concentrations can lead to failure of pregnancy and early embryonic loss. P4 concentrations in serum have been used not only to confirm ovulation and evaluate luteal function but also to monitor the progression of early pregnancy, including the assessment of the risk of ectopic pregnancy. Thus, the determination of serum or plasma P4 concentrations in humans is important not only for the early diagnosis of pregnancy, but also for the evaluation of corpus luteum and placental function during gestation. Many laboratories use radioimmunoassays (RIAs) for the quantification of serum P4, but RIA, although very sensitive, is expensive and requires the availability of specialized laboratories and personnel.

Research Area

SignalTransduction