QuicKey Pro Human Pg(Progesterone) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*10	Inquire	/
96T*5	Inquire	/

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	36.24 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reacitivity	Human
Specificity	This kit recognizes Human Pg in samples.No significant cross-reactivity or interference between Human Pg and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T10 / 96T5
Storage	2-8℃

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Pg are added to the micro ELISA plate wells. Human Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Progesterone (P4) plays a central role in reproductive events associated with the establishment and maintenance of pregnancy in all mammals.4 In the ewe, P4 is synthesized by the corpus luteum during the first 50 d of pregnancy, and then by the placenta until term.6 P4 is needed during early embryonic development for implantation and placentation; insufficient P4 concentrations can lead to failure of pregnancy and early embryonic loss.5 P4 concentrations in plasma have been used not only to determine the pregnancy status of the animal but also to predict the number of fetuses by monitoring the dam in the second half of the pregnancy.8 Thus, the determination of serum or plasma P4 concentrations in sheep is important not only for the early diagnosis of pregnancy, but also to evaluate the number of carried lambs by means of a significant positive correlation with the P4 concentration.2 Many laboratories use radioimmunoassays (RIAs) for the quantification of plasma P4,8,10,11 but RIA, although very sensitive, is expensive and requires the availability of specialized laboratories and personnel.

Protein Alias --TODO	36.24 pg/mL
Research Area	Signal Transduction

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QuicKey Pro Human Pg(Progesterone) ELISA Kit (E-OSEL-H0011)

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