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QuicKey Pro Human E2(Estradiol) ELISA Kit

  • Cat.No.:E-OSEL-H0005

  • Reactivity: Human

To Purchase E-OSEL-H0005

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
Qty:

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 31.25-2000 pg/mL
Sensitivity 13.22 pg/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E2 are added to the micro ELISA plate wells. Human E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T
2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials
2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL
2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL
2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 9.73 25.19 80.21 10.31 25.61 86.43
Standard deviation 0.51 1.26 4.24 0.68 1.19 4.61
CV (%) 5.26 4.99 5.29 6.57 4.66 5.33

Recovery

The recovery of E2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-98 92
EDTA plasma(n=8) 86-101 93
Urine(n=8) 99

Linearity

Samples were spiked with high concentrations of Human E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsOestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol
Research AreaCell biology, Metabolism

Assay Procedures

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Experimental & Molecular Medicine (2020) IF: 5.418
    Melatonin stimulates aromatase expression and estradiol production in human granulosa-lutein cells: relevance for high serum estradiol levels in patients with ovarian hyperstimulation syndrome

    DOI: 10.1038/s12276-020-00491-w

    PMID: 32855437

    Sample: Cell lysate
  2. Nanotoxicology (2018) IF: 5.811
    Metabolomics Reveals the Role of Acetyl-L-Carnitine Metabolism in γ-Fe 2 O 3 NP-induced Embryonic Development Toxicity via Mitochondria Damage

    DOI: 10.1080/17435390.2018.1537411

    PMID: 30663479

    Sample: Serum
  3. Cell Death & Disease (2017) IF: 5.965
    ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats.

    DOI: 10.1038/cddis.2017.494

    PMID: 29072679

    Sample: Serum
  4. Cellular Physiology and Biochemistry (2017) IF: 5.104
    Protective Effects Of Fluvastatin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet Through Enhanced Signaling Of mTOR.

    DOI: 10.1159/000457881

    PMID: 28214901

    Sample: Serum
  5. Journal of Neurochemistry (2018) IF: 4.609
    Evidence of altered depression and dementia-related proteins in the brains of young rats after ovariectomy

    DOI: 10.1111/jnc.14537

    PMID: 29939407

    Sample: Plasma
  6. Journal of Neurochemistry (2018) IF: 4.609
    Evidence of altered depression and dementia‐related proteins in the brains of young rats after ovariectomy.

    DOI: 10.1111/jnc.14537

    PMID: 29939407

    Sample: Serum
  7. Scientific Reports (2017) IF: 4.259
    Diet-Induced Obesity Impairs Spermatogenesis: A Potential Role For Autophagy.

    DOI: 10.1038/srep43475

    PMID: 28276438

    Sample: Serum
  8. Chemosphere (2017) IF: 4.208
    Disruption Of Sex-Hormone Levels And Steroidogenic-Related Gene Expression On Mongolia Racerunner (Eremias Argus) After Exposure To Triadimefon And Its Enantiomers.

    DOI: 10.1016/j.chemosphere.2016.12.096

    PMID: 28039834

    Sample: Plasma
  9. Journal of Biochemical and Molecular Toxicology (2020) IF: 3.606
    Protective roles and mechanisms of rosmarinic acid in cyclophosphamide-induced premature ovarian failure

    DOI: 10.1002/jbt.22591

    PMID: 32711407

    Sample: Serum
  10. Journal of Applied Toxicology (2019) IF: 2.909
    Estrogenic Effects of Fluorinated Diiodine Alkanes in MCF-7 Cells, H295R Cells and Zebrafish Embryo Assays

    DOI: 10.1002/jat.3783

    PMID: 30834569

    Sample: Cell culture medium
  11. IF: 2.852
    Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage
    Sample: Serum
  12. Reproductive Biology and Endocrinology (2018) IF: 2.852
    Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage

    DOI: 10.1186/s12958-018-0404-4

    PMID: 30176887

    Sample: Serum
  13. International Journal of Molecular Medicine (2018) IF: 2.784
    Expression of FKBP52 in the ovaries of PCOS rats

    DOI: 10.3892/ijmm.2018.3998

    PMID: 30483787

    Sample: Serum
  14. Plos One (2017) IF: 2.806
    Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells.

    DOI: 10.1371/journal.pone.0188029

    PMID: 29125859

    Sample: Cell culture supernatant
  15. Journal of Bone And Mineral Metabolism (2017) IF: 2.423
    Investigation Of The Relation Between Bone Mass Density And Serum Preptin Levels In Pre-And Postmenopausal Women.

    DOI: 10.1007/s00774-017-0882-z

    PMID: 29134283

    Sample: Serum
  16. Journal of Assisted Reproduction & Genetics (2015) IF: 2.163
    Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet.

    DOI: 10.1007/s10815-015-0506-2

    PMID: 26081124

    Sample: Serum
  17. Molecular Biology Reports (2020) IF: 1.402
    Acacetin improves endothelial dysfunction and aortic fibrosis in insulin-resistant SHR rats by estrogen receptors

    DOI: 10.1007/s11033-020-05746-3

    PMID: 32892299

    Sample: Serum
  18. Reproduction in Domestic Animals (2018) IF: 1.4
    Methylation pattern variation between goats and rats during the onset of puberty.

    DOI: 10.1111/rda.13172

    PMID: 29577480

  19. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates HighFat DietInduced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    Sample: Serum
  20. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    Sample: Serum
  21. Experimental & Therapeutic Medicine (2015) IF: 1.261
    Electroacupuncture At The Governor Vessel And Bladder Meridian Acupoints Improves Postmenopausal Osteoporosis Through Osteoprotegerin/RANKL/RANK And Wnt/β-Catenin Signaling Pathways.

    DOI: 10.3892/etm.2015.2553

    PMID: 26622351

    Sample: Serum
  22. Preprint (2020)
    Elevated levels of miR-483-5p in oocytes aggravates premature ovarian insufficiency induced by CDDP by targeting the FKBP4

    DOI: 10.21203/rs.3.rs-17131/v1

    Sample: Serum
  23. Medico Legal Update (2020)
    Is Irisin a Phenotypic Parameter in Over-Weight Women with Polycystic Ovary Syndrome?
    Sample: Serum
  24. Journal of Huazhong University of Science and Technology-Medical Sciences (2014) IF: 0.964
    Effects Of Er-Zhi-Wan On Microarchitecture And Regulation Of Wnt/β-Catenin Signaling Pathway In Alveolar Bone Of Ovariectomized Rats.

    DOI: 10.1007/s11596-014-1241-0

    PMID: 24496689

    Sample: Serum
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